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Literature summary extracted from
- Type B nucleoside-diphosphatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity (1988), Eur. J. Biochem., 171, 231-236.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.6 | ADP | 1 mM, 27% inhibition of thiamin-diphosphatase activity, competitive | Rattus norvegicus |  |
| 3.6.1.6 | AMP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
| 3.6.1.6 | ATP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
| 3.6.1.6 | chlorpromazine | 1 mM, 15% inhibition | Rattus norvegicus | |
| 3.6.1.6 | diphosphate | complete inhibition at 1 mM, 61% inhibition at 0.06 mM | Rattus norvegicus | |
| 3.6.1.6 | F- | 100 mM KF, 33% inhibition | Rattus norvegicus | |
| 3.6.1.6 | IMP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
| 3.6.1.6 | NEM | 1 mM, 16% inhibition | Rattus norvegicus | |
| 3.6.1.6 | Promethazine | 1 mM, 15% inhibition | Rattus norvegicus | |
| 3.6.1.6 | pyridoxal 5'-phosphate | 1 mM, 43% inhibition of thiamin-diphosphatase activity, competitive | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.6 | 0.4 | - |
GDP | in presence of Mn2+ | Rattus norvegicus | |
| 3.6.1.6 | 0.54 | - |
IDP | in presence of Mn2+ | Rattus norvegicus | |
| 3.6.1.6 | 0.66 | - |
thiamine diphosphate | in presence of Mn2+ | Rattus norvegicus | |
| 3.6.1.6 | 1.06 | - |
UDP | in presence of Mn2+ | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.6 | microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.6 | Ca2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, TPP, IDP, UDP, CDP | Rattus norvegicus | |
| 3.6.1.6 | Cd2+ | Cd2+ can partially replace Mn2+, 16%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Cd2+, 38% | Rattus norvegicus | |
| 3.6.1.6 | Co2+ | Co2+ can partially replace Mn2+, 20%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Co2+, 55% | Rattus norvegicus | |
| 3.6.1.6 | Manganese | metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit | Rattus norvegicus | |
| 3.6.1.6 | Mg2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, IDP, TPP, UDP, CDP | Rattus norvegicus | |
| 3.6.1.6 | Mn2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: TPP, IDP, GDP, UDP, CDP, dTDP | Rattus norvegicus | |
| 3.6.1.6 | Zinc | metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit | Rattus norvegicus | |
| 3.6.1.6 | Zn2+ | Zn2+ can partially replace Mn2+, 4%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Zn2+, 23% | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 75000 | - |
1 * 75000, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.6 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.6 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.6 | brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 2.43 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.6 | ADP + H2O | no activity | Rattus norvegicus | AMP + phosphate | - |
? | |
| 3.6.1.6 | GDP + H2O | - |
Rattus norvegicus | GMP + phosphate | - |
? | |
| 3.6.1.6 | IDP + H2O | - |
Rattus norvegicus | IMP + phosphate | - |
? | |
| 3.6.1.6 | TPP + H2O | is hydrolyzed twice as efficiently as nucleoside diphosphates | Rattus norvegicus | thiamine phosphate + phosphate | - |
? | |
| 3.6.1.6 | UDP + H2O | - |
Rattus norvegicus | UMP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.6 | monomer | 1 * 75000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.6 | 6.5 | - |
hydrolysis of GDP in presence of Mg2+, TTPase activity in presence of Mg2+ | Rattus norvegicus |
| 3.6.1.6 | 7 | - |
GDPase activity in presence of Mn2+ and Ca2+ | Rattus norvegicus |
| 3.6.1.6 | 7.4 | - |
hydrolysis of thiamin-diphosphate in presence of Mn2+ | Rattus norvegicus |
| 3.6.1.6 | 8 | 8.5 | TTPase activity in presence of Ca2+ | Rattus norvegicus |
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