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Literature summary extracted from

  • Dahlman, N.; Ueckermann, C.
    Properties of four different desoxy-thymidine-5'-triphosphate hydrolyzing enzymes in human serum (1982), Biochem. Int., 5, 185-192.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.39 0.04
-
dTTP
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.39 Ca2+ 1 mM, 70% activation compared with Mn2+ Homo sapiens
3.6.1.39 Mg2+ 1 mM, 80% activation compared with Mn2+ Homo sapiens
3.6.1.39 Mn2+ best activator Homo sapiens
3.6.1.39 Zn2+ 1 mM, very weak activation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.39 dTTP + H2O Homo sapiens
-
dTDP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.39 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.39 partial purification Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.39 serum
-
Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.6.1.39 -80°C, 10 mM Tris/HCl, pH 7,8, 5 mM mercaptoethanol, stable on freezing and thawing two times Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.39 dCTP + H2O 60% activity compared with dTTP as substrate Homo sapiens dCDP + phosphate
-
?
3.6.1.39 dTTP + H2O
-
Homo sapiens dTDP + phosphate
-
?
3.6.1.39 dUTP + H2O 50% activity compared with dTTP as substrate Homo sapiens dUDP + phosphate
-
?
3.6.1.39 UTP + H2O 20% activity compared with dTTP as substrate Homo sapiens UDP + phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.39 7.4 7.6
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.39 6 9 no activity at pH 6, very low activity at pH 9 Homo sapiens