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Literature summary extracted from

  • Roscoe, D.H.
    Thymidine triphosphate nucleotidohydrolase: a phage-induced enzyme in Bacillus subtilis (1969), Virology, 38, 520-526.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.39 dTTP + H2O Bacillus subtilis
-
 dTDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.39 Bacillus subtilis
-
 infected with phage phi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.39 dTTP + H2O
-
 Bacillus subtilis dTMP + diphosphate
-
 ?
3.6.1.39 dTTP + H2O
-
 Bacillus subtilis dTDP + phosphate
-
 ?
3.6.1.39 dUTP + H2O 70% activity compared with dTTP as substrate Bacillus subtilis dUMP + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.39 37
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.39
-
-
 25% of activity compared with activity at 37°C Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.39 50
-
 loss of activity in phage infected cells, no loss of activity in wild type cells Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.39 8.5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.39 7.5 10 measured in Tris-buffer and glycine-buffer Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.39 9
-
 loss of activity above pH 9 Bacillus subtilis