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Literature summary extracted from

  • Keesey, J.K.; Bigelis, R.; Fink, G.R.
    The product of the his4 gene cluster in Saccharomyces cerevisiae. A trifunctional polypeptide (1979), J. Biol. Chem., 254, 7427-7433.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.19 95000
-
 alpha2, 2 * 95000, SDS-PAGE Saccharomyces cerevisiae
3.5.4.19 190000
-
 gel filtration Saccharomyces cerevisiae
3.6.1.31 95000
-
 SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.19 1-(5-phosphoribosyl)-AMP + H2O Saccharomyces cerevisiae
-
 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
-
 ?
3.6.1.31 N-1-(5'-phosphoribosyl)-ATP + H2O Saccharomyces cerevisiae enzyme of histidine biosynthesis pathway N-1-(5'-phosphoribosyl)-AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.19 Saccharomyces cerevisiae
-
-
-
3.6.1.31 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.19
-
 Saccharomyces cerevisiae
3.6.1.31
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.19 903
-
-
 Saccharomyces cerevisiae
3.6.1.31 332
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.19 1-(5-phosphoribosyl)-AMP + H2O
-
 Saccharomyces cerevisiae 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
-
 ?
3.6.1.31 N-1-(5'-phosphoribosyl)-ATP + H2O
-
 Saccharomyces cerevisiae N-1-(5'-phosphoribosyl)-AMP + diphosphate
-
 ?
3.6.1.31 N-1-(5'-phosphoribosyl)-ATP + H2O enzyme of histidine biosynthesis pathway Saccharomyces cerevisiae N-1-(5'-phosphoribosyl)-AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.19 dimer alpha2, 2 * 95000, SDS-PAGE Saccharomyces cerevisiae
3.6.1.31 dimer 2 * 95000, gel filtration, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.19 30
-
 assays at 30°C Saccharomyces cerevisiae