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Literature summary extracted from

  • Nishimune, T.; Hayashi, R.
    Hydrolysis and synthesis of thiamin triphosphate in bacteria (1987), J. Nutr. Sci. Vitaminol., 33, 113-127.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.28 1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-hydroxyethyl)-2-methylpyridinium soluble enzyme: 50% inhibition at 2 mM. Membrane-bound enzyme: no inhibition up to 5 mM Escherichia coli
3.6.1.28 ATP soluble enzyme: no inhibition up to 2 mM, membrane-bound enzyme: 50% inhibition at 5 mM Escherichia coli
3.6.1.28 Ca2+ soluble enzyme: 50% inhibition at 5 mM, membrane-bound enzyme: 20% inhibition at 5 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.28 0.07
-
 thiamine triphosphate membrane-bound enzyme Escherichia coli
3.6.1.28 0.9
-
 thiamine triphosphate soluble enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.28 membrane associated Escherichia coli 16020
-
3.6.1.28 soluble
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.28 Mg2+ soluble enzyme requires Mg2+, enzyme from membrane is Mg2+-independent Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.28 Aneurinibacillus aneurinilyticus
-
-
-
3.6.1.28 Escherichia coli
-
 W 1485
-
3.6.1.28 Escherichia coli W 1485
-
 W 1485
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.28 thiamine triphosphate + H2O
-
 Escherichia coli thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O
-
 Aneurinibacillus aneurinilyticus thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O
-
 Escherichia coli W 1485 thiamine diphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.28 37
-
 membrane-bound enzyme Escherichia coli
3.6.1.28 44
-
 soluble enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.28 6.5
-
 membrane-bound enzyme Escherichia coli
3.6.1.28 7
-
 soluble enzyme Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.28 2
-
 1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-hydroxyethyl)-2-methylpyridinium soluble enzyme Escherichia coli