EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.24 | Li2SO4 | 160% of standard activity at 0.75 M | Escherichia coli | |
3.6.1.24 | NH4Cl | 180% of standard activity at 1 M | Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
3.6.1.24 | inactivation by repeated freezing and thawing | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.24 | alanyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
3.6.1.24 | aminoalkyl adenylate | strongest inhibitor, competitive inhibition of other substrates | Escherichia coli | |
3.6.1.24 | AMP | competitive | Escherichia coli | |
3.6.1.24 | KI | 50% loss of activity at 1 M | Escherichia coli | |
3.6.1.24 | lysyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
3.6.1.24 | Methionyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
3.6.1.24 | SDS | complete loss of activity at 6 mM | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.24 | 0.056 | - |
adenosine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
3.6.1.24 | 0.11 | - |
guanosine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
3.6.1.24 | 0.65 | - |
uridine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
3.6.1.24 | 4 | - |
cytidine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
3.6.1.24 | 770 | - |
thymidine 5'-p-nitrophenylphosphate | - |
Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.24 | additional information | not periplasm | Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.24 | additional information | no metal ions required | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 23000 | - |
sedimentation equilibrium method, gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.24 | p-nitrophenyl 5'-nucleotides + H2O | Escherichia coli | hydrolysis A,C,G,U,T nucleotides | p-nitrophenol + nucleoside phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.24 | Escherichia coli | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.6.1.24 | stable towards reducing agents | Escherichia coli |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.24 | - |
Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.6.1.24 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 0.0018 | - |
crude extract | Escherichia coli |
3.6.1.24 | 6670 | - |
purified enzyme | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.6.1.24 | -20°C, can be stored for at least one year | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.24 | acetyl adenylate + H2O | - |
Escherichia coli | ? | - |
? | |
3.6.1.24 | adenosine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + AMP | - |
? | |
3.6.1.24 | cytidine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + CMP | - |
? | |
3.6.1.24 | guanosine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + GMP | - |
? | |
3.6.1.24 | methionyl adenylate + H2O | - |
Escherichia coli | ? | - |
? | |
3.6.1.24 | p-nitrophenyl 5'-nucleotides + H2O | hydrolysis A,C,G,U,T nucleotides | Escherichia coli | p-nitrophenol + nucleoside phosphate | - |
? | |
3.6.1.24 | thymidine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + TMP | - |
? | |
3.6.1.24 | uridine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + UMP | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 50 | - |
- |
Escherichia coli |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 10 | 65 | rapid loss of activity above 50°C | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | - |
- |
no loss of activity within one day | Escherichia coli |
3.6.1.24 | 65 | - |
loss of activity above 65°C, can be restored by cooling down to 0°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 7.5 | 8.5 | - |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 5.5 | 10.5 | very low activity at pH 5.5 and 10.5 | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.24 | 7.5 | - |
irreversible inactivation below pH 7.5 | Escherichia coli |