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Literature summary extracted from

  • Camacho, A.; Hilgado-Zarco, F.; Bernier-Villamor, V.; Ruiz-PeæRez, L.M.; Gonzalez-Pacanowska, D.
    Properties of Leishmania major dUTP nucleotidohydrolase, a distinct nucleotide-hydrolysing enzyme in kinetoplastids (2000), Biochem. J., 346, 163-168.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 dUDP
-
 Leishmania major
3.6.1.23 dUMP
-
 Leishmania major
3.6.1.23 NaF
-
 Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0045
-
 dUTP
-
 Leishmania major
3.6.1.23 0.17
-
 dUDP
-
 Leishmania major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ dependent on Mg2+ or Mn2+ for optimal activity, maximal activity at 25 mM Mg2+ Leishmania major
3.6.1.23 Mn2+ dependent on Mg2+ or Mn2+ for optimal activity Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 31420
-
 2 * 31420, calculation from amino acid sequence Leishmania major
3.6.1.23 62850
-
 gel filtration Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Leishmania major enzyme is involved in controlling intracellular dUTP levels ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23
-
 Leishmania major

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.23 44.06
-
-
 Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUDP + H2O
-
 Leishmania major dUMP + phosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Leishmania major dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O enzyme is involved in controlling intracellular dUTP levels Leishmania major ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 dimer 2 * 31420, calculation from amino acid sequence Leishmania major