Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Williams, M.V.; Pollack, J.D.
    Purification and characterization of a dUTPase from Acholeplasma laidlawii B-PG9 (1984), J. Bacteriol., 159, 278-282.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 EDTA reversed by Mg2+, Mn2+, Zn2+, Co2+ and Ca2+ Acholeplasma laidlawii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0045
-
 dUTP
-
 Acholeplasma laidlawii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 48000
-
 gel filtration Acholeplasma laidlawii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Acholeplasma laidlawii the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation ?
-
 ?
3.6.1.23 dUTP + H2O Acholeplasma laidlawii B-PG9 the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Acholeplasma laidlawii
-
 B-PG9
-
3.6.1.23 Acholeplasma laidlawii B-PG9
-
 B-PG9
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.23 additional information
-
-
 Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Acholeplasma laidlawii dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Acholeplasma laidlawii B-PG9 dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation Acholeplasma laidlawii ?
-
 ?
3.6.1.23 dUTP + H2O the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation Acholeplasma laidlawii B-PG9 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 55
-
 1 h, stable Acholeplasma laidlawii