Literature summary extracted from
Shlomai, J.; Kornberg, A.
Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA (1978), J. Biol. Chem., 253, 3305-3312.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.6.1.23 |
synthesis |
can reduce uracil incorporation into DNA |
Escherichia coli |
General Stability
EC Number |
General Stability |
Organism |
---|
3.6.1.23 |
stable to freezing and thawing |
Escherichia coli |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
EDTA |
2 mM, complete inhibition |
Escherichia coli |
|
3.6.1.23 |
Mg2+ |
complete inhibition by extensive dialysis against 0.0125 M MgCl2 |
Escherichia coli |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
0.012 |
- |
dUTP |
- |
Escherichia coli |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
Mg2+ |
no requirement for Mg2+ |
Escherichia coli |
|
3.6.1.23 |
Zn2+ |
appears to be essential for activity |
Escherichia coli |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.6.1.23 |
16000 |
- |
4 * 16000, SDS-PAGE |
Escherichia coli |
3.6.1.23 |
64500 |
- |
gel filtration, sucrose density gradient centrifugation |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.1.23 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.6.1.23 |
- |
Escherichia coli |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
3.6.1.23 |
-70°C, stable for at least 5 months |
Escherichia coli |
3.6.1.23 |
0°C, stable for at least 3 months |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
- |
Escherichia coli |
dUMP + diphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.6.1.23 |
tetramer |
4 * 16000, SDS-PAGE |
Escherichia coli |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.6.1.23 |
6.1 |
8 |
pH 5.0: no activity, pH 6.1-7.5: optimum, pH 8.0: about 60% of maximal activity |
Escherichia coli |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
38.3 |
- |
dUTP |
at 30°C |
Escherichia coli |
|
3.6.1.23 |
58.3 |
- |
dUTP |
at 35°C |
Escherichia coli |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.6.1.23 |
6.1 |
7.5 |
- |
Escherichia coli |