BRENDA - Enzyme Database

Cloning, purification, and properties of a novel NADH pyrophosphatase

Frick, D.N.; Bessman, M.J.; J. Biol. Chem. 270, 1529-1534 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.22
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.22
NMNH
competitive inhibitor with Ki: 4.2 mM, no inhibition with NMN+
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.11
-
NADH
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
0.29
-
dNADH
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
0.67
-
AppA
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
1.8
-
ADP-ribose
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
2.6
-
dNAD+
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
5.1
-
NAD+
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.22
Co2+
slight activation
Escherichia coli
3.6.1.22
Fe2+
slight activation
Escherichia coli
3.6.1.22
Mg2+
strong activation
Escherichia coli
3.6.1.22
Mn2+
strong activation
Escherichia coli
3.6.1.22
Zn2+
slight activation
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.22
29774
-
homodimer, 2 * 29774 in SDS-PAGE, gel filtration
Escherichia coli
3.6.1.22
60000
-
gel filtration, Sephadex G-100
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.22
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.22
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.22
more than 98% purity
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.22
ADP-glucose + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
ApppA + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
deamino-NAD+ + H2O
-
209957
Escherichia coli
deamino-NMN + AMP
-
-
-
?
3.6.1.22
deamino-NADH + H2O
-
209957
Escherichia coli
deamino-NMNH + AMP
-
-
-
?
3.6.1.22
FAD + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
NAD+ + H2O
-
209957
Escherichia coli
NMN + AMP
-
209957
Escherichia coli
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209957
Escherichia coli
NMNH + AMP
-
209957
Escherichia coli
ir
3.6.1.22
NADH + H2O
-
209957
Escherichia coli
AMP + NMNH
-
209957
Escherichia coli
?
3.6.1.22
NADP+ + H2O
-
209957
Escherichia coli
NMN + adenosine 3',5'-diphosphate
-
-
-
?
3.6.1.22
NADPH + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209957
Escherichia coli
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.22
dimer
homodimer, 2 * 29774 in SDS-PAGE, gel filtration
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.001
-
NADH
based on two active sites/dimer
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.22
8.5
-
-
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.22
4.1
-
NMNH
competitive inhibitor, no inhibiton with NMN+
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.22
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.22
NMNH
competitive inhibitor with Ki: 4.2 mM, no inhibition with NMN+
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.22
4.1
-
NMNH
competitive inhibitor, no inhibiton with NMN+
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.11
-
NADH
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
0.29
-
dNADH
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
0.67
-
AppA
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
1.8
-
ADP-ribose
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
2.6
-
dNAD+
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
3.6.1.22
5.1
-
NAD+
in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.22
Co2+
slight activation
Escherichia coli
3.6.1.22
Fe2+
slight activation
Escherichia coli
3.6.1.22
Mg2+
strong activation
Escherichia coli
3.6.1.22
Mn2+
strong activation
Escherichia coli
3.6.1.22
Zn2+
slight activation
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.22
29774
-
homodimer, 2 * 29774 in SDS-PAGE, gel filtration
Escherichia coli
3.6.1.22
60000
-
gel filtration, Sephadex G-100
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.22
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.22
more than 98% purity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.22
ADP-glucose + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
ApppA + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
deamino-NAD+ + H2O
-
209957
Escherichia coli
deamino-NMN + AMP
-
-
-
?
3.6.1.22
deamino-NADH + H2O
-
209957
Escherichia coli
deamino-NMNH + AMP
-
-
-
?
3.6.1.22
FAD + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
NAD+ + H2O
-
209957
Escherichia coli
NMN + AMP
-
209957
Escherichia coli
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209957
Escherichia coli
NMNH + AMP
-
209957
Escherichia coli
ir
3.6.1.22
NADH + H2O
-
209957
Escherichia coli
AMP + NMNH
-
209957
Escherichia coli
?
3.6.1.22
NADP+ + H2O
-
209957
Escherichia coli
NMN + adenosine 3',5'-diphosphate
-
-
-
?
3.6.1.22
NADPH + H2O
-
209957
Escherichia coli
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209957
Escherichia coli
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.22
dimer
homodimer, 2 * 29774 in SDS-PAGE, gel filtration
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.001
-
NADH
based on two active sites/dimer
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.22
8.5
-
-
Escherichia coli