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Literature summary extracted from

  • Frick, D.N.; Bessman, M.J.
    Cloning, purification, and properties of a novel NADH pyrophosphatase (1995), J. Biol. Chem., 270, 1529-1534.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.22
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.22 NMNH competitive inhibitor with Ki: 4.2 mM, no inhibition with NMN+ Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.22 0.11
-
 NADH in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
3.6.1.22 0.29
-
 deamino-NADH in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
3.6.1.22 0.67
-
 AppA in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
3.6.1.22 1.8
-
 ADP-ribose in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
3.6.1.22 2.6
-
 deamino-NAD+ in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli
3.6.1.22 5.1
-
 NAD+ in presence of 20 mM MgCl2, 50 mM Tris-HCl, pH 8.5 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.22 Co2+ slight activation Escherichia coli
3.6.1.22 Fe2+ slight activation Escherichia coli
3.6.1.22 Mg2+ strong activation Escherichia coli
3.6.1.22 Mn2+ strong activation Escherichia coli
3.6.1.22 Zn2+ slight activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.22 29774
-
 homodimer, 2 * 29774 in SDS-PAGE, gel filtration Escherichia coli
3.6.1.22 60000
-
 gel filtration, Sephadex G-100 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.22 NADH + H2O Escherichia coli possible function in regulation of NAD+/NADH-ratio NMNH + AMP
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.22 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.22 more than 98% purity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.22 ADP-glucose + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 ADPribose + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 AppA + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 ApppA + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 deamino-NAD+ + H2O
-
 Escherichia coli deamino-NMN + AMP
-
 ?
3.6.1.22 deamino-NADH + H2O
-
 Escherichia coli deamino-NMNH + AMP
-
 ?
3.6.1.22 FAD + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 NAD+ + H2O
-
 Escherichia coli NMN + AMP
-
 ir
3.6.1.22 NADH + H2O possible function in regulation of NAD+/NADH-ratio Escherichia coli NMNH + AMP
-
 ir
3.6.1.22 NADH + H2O
-
 Escherichia coli AMP + NMNH
-
 ?
3.6.1.22 NADP+ + H2O
-
 Escherichia coli NMN + adenosine 3',5'-diphosphate
-
 ?
3.6.1.22 NADPH + H2O
-
 Escherichia coli ?
-
 ?
3.6.1.22 P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.22 dimer homodimer, 2 * 29774 in SDS-PAGE, gel filtration Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.22 0.001
-
 NADH based on two active sites/dimer Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.22 8.5
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.22 4.1
-
 NMNH competitive inhibitor, no inhibiton with NMN+ Escherichia coli