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Literature summary extracted from

  • Davies, R.; King, H.K.
    An enzyme degrading reduced nicotinamide-adenine dinucleotide in Proteus vulgaris (1978), Biochem. J., 175, 669-674.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.22 Urea 8 M urea, 30 mM Phosphate buffer, pH 7.4, 100% activation Proteus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.22 Al3+
-
 Proteus vulgaris
3.6.1.22 Ba2+
-
 Proteus vulgaris
3.6.1.22 Cr2+
-
 Proteus vulgaris
3.6.1.22 Fe2+
-
 Proteus vulgaris
3.6.1.22 Sn2+
-
 Proteus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.22 Co2+ necessary for maximum activity Proteus vulgaris
3.6.1.22 Mg2+ strong activation at concentration of 0.4 mM Proteus vulgaris
3.6.1.22 Mn2+ strong activation at concentration of 0.4 mM Proteus vulgaris
3.6.1.22 Ni2+ strong activation at concentration of 0.4 mM Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.22 Proteus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.22
-
 Proteus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.6.1.22 18°C, 50 mM phosphate buffer, pH 7.0, 1 h, 70% loss of activity Proteus vulgaris
3.6.1.22 4°C, 50 mM phosphate buffer, pH 7.0, overnight, 70% loss of activity Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.22 NAD+ + H2O
-
 Proteus vulgaris NMN + AMP
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.22 73
-
 2 min causes activation Proteus vulgaris
3.6.1.22 98
-
 few min at 98°C causes loss of activity, 30 s causes activation Proteus vulgaris