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Literature summary extracted from

  • Byrd, J.C.; Fearney, F.J.; Kim, Y.S.
    Rat intestinal nucleotide-sugar pyrophosphatase. Localization, partial purification, and substrate specificity (1985), J. Biol. Chem., 260, 7474-7480.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 2,3-dimercapto-1-propanol complete inhibition at 5 mM Rattus sp.
3.6.1.9 Adenyl-5'-yl imidodiphosphate 61-78% inhibition at 1 mM Rattus sp.
3.6.1.9 alpha,beta-methylene adenosine 5'-triphosphate 61-78% inhibition at 0.3 mM Rattus sp.
3.6.1.9 CDP-choline 62% inhibition at 0.2 mM Rattus sp.
3.6.1.9 coenzyme A 90% inhibition at 0.2 mM Rattus sp.
3.6.1.9 EDTA inactivation after 30 min preincubation at 6 mM Rattus sp.
3.6.1.9 FAD+ 79% inhibition at 0.2 mM Rattus sp.
3.6.1.9 NAD+ 82% inhibition at 0.2 mM Rattus sp.
3.6.1.9 p-nitrophenyl 3'-thymidylate 15% inhibition at 0.2 mM Rattus sp.
3.6.1.9 p-nitrophenyl 5'-thymidylate 15% inhibition at 0.2 mM Rattus sp.
3.6.1.9 thiamine diphosphate 17% inhibition at 0.2 mM Rattus sp.
3.6.1.9 UDP-glucose competitive inhibition of phosphodiesterase activity Rattus sp.
3.6.1.18 CDP-choline
-
 Rattus norvegicus
3.6.1.18 CoA
-
 Rattus norvegicus
3.6.1.18 EDTA irreversible inhibition Rattus norvegicus
3.6.1.18 GDP-Man inhibits FAD diphosphatase activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.04
-
 GDP-mannose
-
 Rattus sp.
3.6.1.9 0.21
-
 p-nitrophenyl 5'-TMP
-
 Rattus sp.
3.6.1.9 1.23
-
 FAD
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.9 brush border
-
 Rattus sp. 5903
-
3.6.1.18 brush border
-
 Rattus norvegicus 5903
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.18 135000
-
 SDS-PAGE of papain-solubilized enzyme Rattus norvegicus
3.6.1.18 220000
-
 SDS-PAGE of detergent-solubilized enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 FAD + H2O Rattus sp.
-
 FMN + AMP
-
 ?
3.6.1.9 GDP-mannose + H2O Rattus sp.
-
 GMP + D-mannose-1-phosphate
-
 ?
3.6.1.18 FAD + H2O Rattus norvegicus
-
 FMN + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Rattus sp.
-
-
-
3.6.1.18 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 partial Rattus sp.
3.6.1.18
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 intestine
-
 Rattus sp.
-
3.6.1.18 intestinal mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 4.7
-
-
 Rattus sp.
3.6.1.18 0.458
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 FAD + H2O
-
 Rattus sp. FMN + AMP
-
 ?
3.6.1.9 GDP-mannose + H2O
-
 Rattus sp. GMP + D-mannose-1-phosphate
-
 ?
3.6.1.9 p-nitrophenyl-dTMP + H2O
-
 Rattus sp. p-nitrophenol + dTMP
-
 ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
3.6.1.18 FAD + H2O
-
 Rattus norvegicus FMN + AMP
-
 ?
3.6.1.18 GDP-Man + H2O EC 3.6.1.9 activity Rattus norvegicus Man-1-phosphate + GMP
-
 ?
3.6.1.18 nucleotide sugars + H2O EC 3.6.1.9 activity Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.9 65
-
 half-life: 6.4-10.4 min Rattus sp.
3.6.1.18 55
-
 no inactivation within 120 min Rattus norvegicus
3.6.1.18 65
-
 inactivates Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 7 8.5
-
 Rattus sp.
3.6.1.18 7 8.5
-
 Rattus norvegicus