BRENDA - Enzyme Database

Isolation and charcterization of diadenosine tetraphosphate (Ap4A) hydrolase from Schizosaccharomyces pombe

Robinson, A.K.; de la Pena C.E.; Barnes, L.D.; Biochim. Biophys. Acta 1161, 139-148 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.17
beta-mercaptoethanol
slight activation
Saccharomyces cerevisiae
3.6.1.17
dithiothreitol
slight activation
Saccharomyces cerevisiae
3.6.1.17
phosphate
activating below 30 mM
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
fluoride
no effect
Saccharomyces cerevisiae
3.6.1.17
phosphate
inhibitory above 30 mM
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
22000
-
SDS-PAGE
Saccharomyces cerevisiae
3.6.1.17
49000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Saccharomyces cerevisiae
-
ATP + AMP
-
Saccharomyces cerevisiae
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Saccharomyces cerevisiae
-
GTP + GMP
-
Saccharomyces cerevisiae
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Saccharomyces cerevisiae
-
Schizosaccharomyces pombe
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209913
Saccharomyces cerevisiae
ATP + AMP
-
209913
Saccharomyces cerevisiae
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209913
Saccharomyces cerevisiae
GTP + GMP
-
209913
Saccharomyces cerevisiae
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.6.1.17
beta-mercaptoethanol
slight activation
Saccharomyces cerevisiae
3.6.1.17
dithiothreitol
slight activation
Saccharomyces cerevisiae
3.6.1.17
phosphate
activating below 30 mM
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
fluoride
no effect
Saccharomyces cerevisiae
3.6.1.17
phosphate
inhibitory above 30 mM
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
22000
-
SDS-PAGE
Saccharomyces cerevisiae
3.6.1.17
49000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Saccharomyces cerevisiae
-
ATP + AMP
-
Saccharomyces cerevisiae
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Saccharomyces cerevisiae
-
GTP + GMP
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209913
Saccharomyces cerevisiae
ATP + AMP
-
209913
Saccharomyces cerevisiae
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209913
Saccharomyces cerevisiae
GTP + GMP
-
209913
Saccharomyces cerevisiae
?