BRENDA - Enzyme Database

The green alga Scenedesmus obliquus contains both diadenosine 5',5'''-P1,P4-tetraphosphate (asymmetrical) pyrophosphohyrolase and phosphorylase activities

McLennan, A.G.; Mayers, E.; Hankin, S.; Thorne, N.M.; Prescott, M.; Powls, R.; Biochem. J. 300, 183-189 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
additional information
no inhibition by F- or adenosine 5'-tetraphosphate
Tetradesmus obliquus
3.6.1.17
fluoride
-
Chlorella vulgaris
3.6.1.17
fluoride
-
Scenedesmus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.0053
-
P1,P4-bis(5'-adenosyl) tetraphosphate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.07
-
vanadate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.13
-
arsenate
P1,P4-bis(5'-adenosyl) tetraphosphate-arsenolysis, pH 7.4, 25C
Tetradesmus obliquus
2.7.7.53
0.16
-
phosphate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.45
-
molybdate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.52
-
chromate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
1.75
-
tungstate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
as good as Mn2+; less effective than Co2+; less effective than Mg2+
Tetradesmus obliquus
2.7.7.53
Cd2+
slight activation
Tetradesmus obliquus
2.7.7.53
Co2+
activation, less effective than Mn2+ or Ca2+ or Mg2+
Tetradesmus obliquus
2.7.7.53
divalent metal cations
requirement
Tetradesmus obliquus
2.7.7.53
Mg2+
-
Tetradesmus obliquus
2.7.7.53
Mn2+
10 mM; less effective than Mg2+ or Co2+
Tetradesmus obliquus
2.7.7.53
Zn2+
slight activation
Tetradesmus obliquus
3.6.1.17
Mg2+
-
Chlorella vulgaris
3.6.1.17
Mg2+
-
Scenedesmus sp.
3.6.1.17
Mn2+
-
Chlorella vulgaris
3.6.1.17
Mn2+
-
Scenedesmus sp.
3.6.1.17
Zn2+
strongest activation
Chlorella vulgaris
3.6.1.17
Zn2+
strongest activation
Scenedesmus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
46000
48000
gel filtration
Tetradesmus obliquus
2.7.7.53
46000
-
1 * 46000, SDS-PAGE
Tetradesmus obliquus
3.6.1.17
20000
26000
gel filtration, MW depends on subspecies
Scenedesmus sp.
3.6.1.17
22000
-
gel filtration
Chlorella vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Chlorella vulgaris
-
ATP + AMP
-
Chlorella vulgaris
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Scenedesmus sp.
-
ATP + AMP
-
Scenedesmus sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Chlorella vulgaris
-
green alga
-
2.7.7.53
Scenedesmus basiliensis
-
green alga
-
2.7.7.53
Scenedesmus quadricauda
-
green alga
-
2.7.7.53
Tetradesmus obliquus
-
green alga
-
3.6.1.17
Chlorella vulgaris
-
-
-
3.6.1.17
Scenedesmus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold
Tetradesmus obliquus
3.6.1.17
-
Chlorella vulgaris
3.6.1.17
-
Scenedesmus sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.7.53
0.00014
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus quadricauda
2.7.7.53
0.00065
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
2.7.7.53
0.00096
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Chlorella vulgaris
2.7.7.53
0.0012
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus basiliensis
2.7.7.53
1.96
-
purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
Storage Stability
EC Number
Storage Stability
Organism
2.7.7.53
-20C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable
Tetradesmus obliquus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
ADP + arsenate
-
209909
Tetradesmus obliquus
AMP + phosphate + ?
-
209909
Tetradesmus obliquus
?
2.7.7.53
ADP + ATP
-
209909
Tetradesmus obliquus
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Scenedesmus basiliensis
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Chlorella vulgaris
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Scenedesmus quadricauda
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + phosphate
ADP/phosphate exchange reaction
209909
Tetradesmus obliquus
phosphate + ADP
-
209909
Tetradesmus obliquus
r
2.7.7.53
additional information
no substrates are AMP, p-nitrophenylthymidine 5'-triphosphate, bis-p-nitrophenyl phosphate
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Scenedesmus basiliensis
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Chlorella vulgaris
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Scenedesmus quadricauda
?
-
-
-
-
2.7.7.53
additional information
no substrates are ADP, ATP, NAD+
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
no activity with
209909
Tetradesmus obliquus
ADP
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Tetradesmus obliquus
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Scenedesmus basiliensis
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Chlorella vulgaris
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Scenedesmus quadricauda
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Tetradesmus obliquus
ADP + ATP
-
209909
Tetradesmus obliquus
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Scenedesmus basiliensis
ADP + ATP
-
209909
Scenedesmus basiliensis
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Chlorella vulgaris
ADP + ATP
-
209909
Chlorella vulgaris
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Scenedesmus quadricauda
ADP + ATP
-
209909
Scenedesmus quadricauda
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
209909
Tetradesmus obliquus
?
-
-
-
r
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209909
Chlorella vulgaris
ATP + AMP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209909
Scenedesmus sp.
ATP + AMP
-
209909
Scenedesmus sp.
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209909
Chlorella vulgaris
ADP + ATP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209909
Scenedesmus sp.
ADP + ATP
-
209909
Scenedesmus sp.
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209909
Chlorella vulgaris
2 ATP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209909
Scenedesmus sp.
2 ATP
-
209909
Scenedesmus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 46000, SDS-PAGE
Tetradesmus obliquus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Chlorella vulgaris
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus basiliensis
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus quadricauda
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
2.7.7.53
30
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at
Tetradesmus obliquus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
5.3
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at
Tetradesmus obliquus
2.7.7.53
7.5
9.5
-
Tetradesmus obliquus
2.7.7.53
8
-
assay at
Chlorella vulgaris
2.7.7.53
8
-
assay at
Scenedesmus basiliensis
2.7.7.53
8
-
assay at
Scenedesmus quadricauda
2.7.7.53
8
-
assay at
Tetradesmus obliquus
3.6.1.17
9
-
-
Chlorella vulgaris
3.6.1.17
9
-
-
Scenedesmus sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.53
6.5
9.8
about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8
Tetradesmus obliquus
3.6.1.17
6.5
9.8
35% activity at pH 6.5 and 80% activity at pH 9.8
Chlorella vulgaris
3.6.1.17
6.5
9.8
35% activity at pH 6.5 and 80% activity at pH 9.8
Scenedesmus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
additional information
no inhibition by F- or adenosine 5'-tetraphosphate
Tetradesmus obliquus
3.6.1.17
fluoride
-
Chlorella vulgaris
3.6.1.17
fluoride
-
Scenedesmus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.0053
-
P1,P4-bis(5'-adenosyl) tetraphosphate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.07
-
vanadate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.13
-
arsenate
P1,P4-bis(5'-adenosyl) tetraphosphate-arsenolysis, pH 7.4, 25C
Tetradesmus obliquus
2.7.7.53
0.16
-
phosphate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.45
-
molybdate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
0.52
-
chromate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
2.7.7.53
1.75
-
tungstate
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis, pH 8.0, 25C
Tetradesmus obliquus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
as good as Mn2+; less effective than Co2+; less effective than Mg2+
Tetradesmus obliquus
2.7.7.53
Cd2+
slight activation
Tetradesmus obliquus
2.7.7.53
Co2+
activation, less effective than Mn2+ or Ca2+ or Mg2+
Tetradesmus obliquus
2.7.7.53
divalent metal cations
requirement
Tetradesmus obliquus
2.7.7.53
Mg2+
-
Tetradesmus obliquus
2.7.7.53
Mn2+
10 mM; less effective than Mg2+ or Co2+
Tetradesmus obliquus
2.7.7.53
Zn2+
slight activation
Tetradesmus obliquus
3.6.1.17
Mg2+
-
Chlorella vulgaris
3.6.1.17
Mg2+
-
Scenedesmus sp.
3.6.1.17
Mn2+
-
Chlorella vulgaris
3.6.1.17
Mn2+
-
Scenedesmus sp.
3.6.1.17
Zn2+
strongest activation
Chlorella vulgaris
3.6.1.17
Zn2+
strongest activation
Scenedesmus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
46000
48000
gel filtration
Tetradesmus obliquus
2.7.7.53
46000
-
1 * 46000, SDS-PAGE
Tetradesmus obliquus
3.6.1.17
20000
26000
gel filtration, MW depends on subspecies
Scenedesmus sp.
3.6.1.17
22000
-
gel filtration
Chlorella vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Chlorella vulgaris
-
ATP + AMP
-
Chlorella vulgaris
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Scenedesmus sp.
-
ATP + AMP
-
Scenedesmus sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis: 2600fold, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis: 8531fold
Tetradesmus obliquus
3.6.1.17
-
Chlorella vulgaris
3.6.1.17
-
Scenedesmus sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.7.53
0.00014
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus quadricauda
2.7.7.53
0.00065
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
2.7.7.53
0.00096
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Chlorella vulgaris
2.7.7.53
0.0012
-
crude cell extract, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus basiliensis
2.7.7.53
1.96
-
purified enzyme, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.7.53
-20C, 30 mM HEPES/KOH buffer, pH 7.8, 50% glycerol, stable
Tetradesmus obliquus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
ADP + arsenate
-
209909
Tetradesmus obliquus
AMP + phosphate + ?
-
209909
Tetradesmus obliquus
?
2.7.7.53
ADP + ATP
-
209909
Tetradesmus obliquus
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Scenedesmus basiliensis
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Chlorella vulgaris
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + ATP
-
209909
Scenedesmus quadricauda
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
-
-
r
2.7.7.53
ADP + phosphate
ADP/phosphate exchange reaction
209909
Tetradesmus obliquus
phosphate + ADP
-
209909
Tetradesmus obliquus
r
2.7.7.53
additional information
no substrates are AMP, p-nitrophenylthymidine 5'-triphosphate, bis-p-nitrophenyl phosphate
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Scenedesmus basiliensis
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Chlorella vulgaris
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
209909
Scenedesmus quadricauda
?
-
-
-
-
2.7.7.53
additional information
no substrates are ADP, ATP, NAD+
209909
Tetradesmus obliquus
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
no activity with
209909
Tetradesmus obliquus
ADP
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Tetradesmus obliquus
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Scenedesmus basiliensis
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Chlorella vulgaris
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
-
209909
Scenedesmus quadricauda
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Tetradesmus obliquus
ADP + ATP
-
209909
Tetradesmus obliquus
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Scenedesmus basiliensis
ADP + ATP
-
209909
Scenedesmus basiliensis
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Chlorella vulgaris
ADP + ATP
-
209909
Chlorella vulgaris
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
209909
Scenedesmus quadricauda
ADP + ATP
-
209909
Scenedesmus quadricauda
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + tungstate
-
209909
Tetradesmus obliquus
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
209909
Tetradesmus obliquus
?
-
-
-
r
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209909
Chlorella vulgaris
ATP + AMP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209909
Scenedesmus sp.
ATP + AMP
-
209909
Scenedesmus sp.
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209909
Chlorella vulgaris
ADP + ATP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209909
Scenedesmus sp.
ADP + ATP
-
209909
Scenedesmus sp.
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209909
Chlorella vulgaris
2 ATP
-
209909
Chlorella vulgaris
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209909
Scenedesmus sp.
2 ATP
-
209909
Scenedesmus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 46000, SDS-PAGE
Tetradesmus obliquus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Chlorella vulgaris
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus basiliensis
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Scenedesmus quadricauda
2.7.7.53
25
-
assay at, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Tetradesmus obliquus
2.7.7.53
30
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at
Tetradesmus obliquus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
5.3
-
P1,P4-bis(5'-adenosyl) tetraphosphate-synthesis assay at
Tetradesmus obliquus
2.7.7.53
7.5
9.5
-
Tetradesmus obliquus
2.7.7.53
8
-
assay at
Chlorella vulgaris
2.7.7.53
8
-
assay at
Scenedesmus basiliensis
2.7.7.53
8
-
assay at
Scenedesmus quadricauda
2.7.7.53
8
-
assay at
Tetradesmus obliquus
3.6.1.17
9
-
-
Chlorella vulgaris
3.6.1.17
9
-
-
Scenedesmus sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.53
6.5
9.8
about 20% of maximal activity at pH 6.5 and about 80% of maximal activity at pH 9.8
Tetradesmus obliquus
3.6.1.17
6.5
9.8
35% activity at pH 6.5 and 80% activity at pH 9.8
Chlorella vulgaris
3.6.1.17
6.5
9.8
35% activity at pH 6.5 and 80% activity at pH 9.8
Scenedesmus sp.