BRENDA - Enzyme Database

Dinucleoside tetraphosphatase from human blood cells: Purification and characterization as a high specific activity enzyme recognized by an anti-rat tetraphosphatase antibody

Pinto, R.M.; Costas, M.J.; Fernandez, A.; Canales, J.; Garcia-Agundez, J.A.; Camselle, J.C.; FEBS Lett. 287, 85-88 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive; Ki in nanomolar range
Homo sapiens
3.6.1.17
adenosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
3.6.1.17
fluoride
-
Homo sapiens
3.6.1.17
fluoride
-
Rattus norvegicus
3.6.1.17
Zn2+
-
Homo sapiens
3.6.1.17
Zn2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0007
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
required, optimum concentration 3-10 mM
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
18000
-
SDS-PAGE, immunoblot
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Homo sapiens
-
ATP + AMP
-
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Rattus norvegicus
-
ATP + AMP
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Homo sapiens
-
-
-
3.6.1.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.17
blood
-
Homo sapiens
-
3.6.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.0001
-
crude extract
Homo sapiens
3.6.1.17
18.5
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209906
Homo sapiens
ATP + AMP
-
209906
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209906
Rattus norvegicus
ATP + AMP
-
209906
Rattus norvegicus
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive; Ki in nanomolar range
Homo sapiens
3.6.1.17
adenosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
3.6.1.17
fluoride
-
Homo sapiens
3.6.1.17
fluoride
-
Rattus norvegicus
3.6.1.17
Zn2+
-
Homo sapiens
3.6.1.17
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0007
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
required, optimum concentration 3-10 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
18000
-
SDS-PAGE, immunoblot
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Homo sapiens
-
ATP + AMP
-
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Rattus norvegicus
-
ATP + AMP
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.17
blood
-
Homo sapiens
-
3.6.1.17
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.0001
-
crude extract
Homo sapiens
3.6.1.17
18.5
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209906
Homo sapiens
ATP + AMP
-
209906
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209906
Rattus norvegicus
ATP + AMP
-
209906
Rattus norvegicus
?