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Literature summary extracted from
- Lanterns of firefly Photinus pyralis contain abundant diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase activity (1995), J. Biol. Chem., 270, 3706-3709.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.17 | adenosine 5'-tetraphosphate | competitive; Ki in nanomolar range | Photinus pyralis |  |
| 3.6.1.17 | fluoride | Ki: 0.05; non-competitive | Photinus pyralis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.17 | 0.0019 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Photinus pyralis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.17 | 14500 | - |
gel filtration | Photinus pyralis |
| 3.6.1.17 | 16000 | - |
SDS-PAGE | Photinus pyralis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | Photinus pyralis | possible role as reporter for luciferase gene | ATP + AMP | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.17 | Photinus pyralis | - |
firefly | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.17 | - |
Photinus pyralis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.17 | 0.02 | - |
crude extract | Photinus pyralis |
| 3.6.1.17 | 13.5 | - |
purified enzyme | Photinus pyralis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Photinus pyralis | ATP + AMP | - |
? | |
| 3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | possible role as reporter for luciferase gene | Photinus pyralis | ATP + AMP | - |
? | |
| 3.6.1.17 | P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Photinus pyralis | ADP + ATP | - |
? | |
| 3.6.1.17 | P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Photinus pyralis | 2 ATP | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.17 | monomer | 1 * 16000, SDS-PAGE, gel filtration | Photinus pyralis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.17 | 3.6 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Photinus pyralis | |
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