BRENDA - Enzyme Database

Lanterns of firefly Photinus pyralis contain abundant diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase activity

McLennan, A.G.; Mayers, E.; Walker-Smith, I.; Chen, H.; J. Biol. Chem. 270, 3706-3709 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive; Ki in nanomolar range
Photinus pyralis
3.6.1.17
fluoride
Ki: 0.05; non-competitive
Photinus pyralis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0019
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Photinus pyralis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
14500
-
gel filtration
Photinus pyralis
3.6.1.17
16000
-
SDS-PAGE
Photinus pyralis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Photinus pyralis
possible role as reporter for luciferase gene
ATP + AMP
-
Photinus pyralis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Photinus pyralis
-
firefly
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
-
Photinus pyralis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.02
-
crude extract
Photinus pyralis
3.6.1.17
13.5
-
purified enzyme
Photinus pyralis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209905
Photinus pyralis
ATP + AMP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
possible role as reporter for luciferase gene
209905
Photinus pyralis
ATP + AMP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209905
Photinus pyralis
ADP + ATP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209905
Photinus pyralis
2 ATP
-
209905
Photinus pyralis
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.17
monomer
1 * 16000, SDS-PAGE, gel filtration
Photinus pyralis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
3.6
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Photinus pyralis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
competitive; Ki in nanomolar range
Photinus pyralis
3.6.1.17
fluoride
Ki: 0.05; non-competitive
Photinus pyralis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0019
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Photinus pyralis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
14500
-
gel filtration
Photinus pyralis
3.6.1.17
16000
-
SDS-PAGE
Photinus pyralis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Photinus pyralis
possible role as reporter for luciferase gene
ATP + AMP
-
Photinus pyralis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
-
Photinus pyralis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.02
-
crude extract
Photinus pyralis
3.6.1.17
13.5
-
purified enzyme
Photinus pyralis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209905
Photinus pyralis
ATP + AMP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
possible role as reporter for luciferase gene
209905
Photinus pyralis
ATP + AMP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209905
Photinus pyralis
ADP + ATP
-
209905
Photinus pyralis
?
3.6.1.17
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O
-
209905
Photinus pyralis
2 ATP
-
209905
Photinus pyralis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.17
monomer
1 * 16000, SDS-PAGE, gel filtration
Photinus pyralis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
3.6
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Photinus pyralis