BRENDA - Enzyme Database

Diguanosinetetraphosphatase from rat liver: Activity on diadenosine tetraphosphate and inhibition by adenosine tetraphosphate

Lobaton, C.D.; Vallejo, C.G.; Sillero, A.; Sillero, M.A.G.; Eur. J. Biochem. 50, 495-501 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
3.6.1.17
guanosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.002
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Rattus norvegicus
3.6.1.17
0.002
-
P1,P4-bis(5'-guanosyl) tetraphosphate
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
required, maximum activity at 5 mM
Rattus norvegicus
3.6.1.17
Mn2+
15% of maximum activity
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
22000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Rattus norvegicus
-
ATP + AMP
-
Rattus norvegicus
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Rattus norvegicus
-
GTP + GMP
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
partial
Rattus norvegicus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.002
-
crude extract
Rattus norvegicus
3.6.1.17
0.436
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209895
Rattus norvegicus
ATP + AMP
-
209895
Rattus norvegicus
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209895
Rattus norvegicus
GTP + GMP
-
209895
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
8
-
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
3.6.1.17
guanosine 5'-tetraphosphate
Ki in nanomolar range
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.002
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Rattus norvegicus
3.6.1.17
0.002
-
P1,P4-bis(5'-guanosyl) tetraphosphate
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Mg2+
required, maximum activity at 5 mM
Rattus norvegicus
3.6.1.17
Mn2+
15% of maximum activity
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
22000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Rattus norvegicus
-
ATP + AMP
-
Rattus norvegicus
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Rattus norvegicus
-
GTP + GMP
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
partial
Rattus norvegicus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.002
-
crude extract
Rattus norvegicus
3.6.1.17
0.436
-
partially purified enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209895
Rattus norvegicus
ATP + AMP
-
209895
Rattus norvegicus
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209895
Rattus norvegicus
GTP + GMP
-
209895
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
8
-
-
Rattus norvegicus