Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lorenz, B.; Batel, R.; Bachinski, N.; Mueller, W.E.G.; Schroeder, H.C.
    Purification and characterization of two exopolyphosphatases from the marine sponge Tethya lyncurium (1995), Biochim. Biophys. Acta, 1245, 17-28.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.11 repeated freezing and thawing destroys activity Tethya aurantium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 ADP 0.5 mM, 30% loss of activity, exopolyphosphatase II Tethya aurantium
3.6.1.11 ATP 0.5 mM, 47% loss of activity, exopolyphosphatase II Tethya aurantium
3.6.1.11 EDTA exopolyphosphatase II Tethya aurantium
3.6.1.11 Mg2+ exopolyphosphatase I Tethya aurantium
3.6.1.11 NaCl 600 mM NaCl, 73% inhibition of exopolyphosphatase I and 84% inhibition of exopolyphosphatase II Tethya aurantium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 0.0092
-
 polyP10 exopolyphosphatase II Tethya aurantium
3.6.1.11 0.0238
-
 polyP10 exopolyphosphatase I Tethya aurantium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Mg2+ optimal activity of exopolyphosphatase II in presence of 3 mM Tethya aurantium
3.6.1.11 additional information exopolyphosphatase I does not require divalent cations Tethya aurantium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 45000
-
 exopolyphosphatase I, gel filtration Tethya aurantium
3.6.1.11 70000
-
 exopolyphosphatase II, gel filtration Tethya aurantium
3.6.1.11 73000
-
 1 * 73000, exopolyphosphatase I, SDS-PAGE Tethya aurantium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O Tethya aurantium the inhibitory effect of long-chain polyphosphates on adenylate kinase is higher than that of short-chain polyphosphates, suggesting a potential role of polyphosphate metabolism in regulating intracellular concentration of adenylate nucleotides (polyphosphate)n-1 + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Tethya aurantium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11 exopolyphosphatase I and II Tethya aurantium

Storage Stability

EC Number Storage Stability Organism
3.6.1.11 -20°C, in 50 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl2, 0.5 mM EDTA, 50 mM NaCl, 0.1% w/v bovine serum albumin, 40% v/v glycerol, exopolyphosphatase I and II, less than 30% loss of activity after 2 months Tethya aurantium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O
-
 Tethya aurantium (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 (polyphosphate)n + H2O the inhibitory effect of long-chain polyphosphates on adenylate kinase is higher than that of short-chain polyphosphates, suggesting a potential role of polyphosphate metabolism in regulating intracellular concentration of adenylate nucleotides Tethya aurantium (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 polyP10 + H2O
-
 Tethya aurantium polyP9 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 monomer 1 * 45000, exopolyphosphatase I, SDS-PAGE Tethya aurantium
3.6.1.11 monomer 1 * 73000, exopolyphosphatase I, SDS-PAGE Tethya aurantium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 5
-
 exopolyphosphatase I Tethya aurantium
3.6.1.11 7.5
-
 exopolyphosphatase II Tethya aurantium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.11 4.6 5.4 pH 4.6: 81% of maximal activity, pH 5.4: 59% of maximal activity, exopolyphosphatase I Tethya aurantium
3.6.1.11 7 9 pH 7.0: 5% of maximal activity, pH 9.0: 57% of maximal activity, exopolyphosphatase I Tethya aurantium