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Literature summary extracted from

  • Andreeva, N.A.; Kulakovskaya, T.V.; Kualev, I.S.
    Purification and properties of exopolyphosphatase isolated from Saccharomyces cerevisiae vacuoles (1998), FEBS Lett., 429, 194-196.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.11 arginine stimulates Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 diphosphate 1 mM, 48% inhibition Saccharomyces cerevisiae
3.6.1.11 EDTA
-
 Saccharomyces cerevisiae
3.6.1.11 heparin
-
 Saccharomyces cerevisiae
3.6.1.11 iodoacetamide 10 mM, 2% inhibition Saccharomyces cerevisiae
3.6.1.11 Mg2+ 0.05 mM, 30% inhibition in a reaction mixture containing Co2+ at its optimal concentration Saccharomyces cerevisiae
3.6.1.11 NaF 10 mM, 20% inhibition Saccharomyces cerevisiae
3.6.1.11 tripolyphosphate 1 mM, 62% inhibition Saccharomyces cerevisiae
3.6.1.11 Zn2+ 0.05 mM, 71% inhibition in a reaction mixture containing Co2+ at its optimal concentration Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 0.0024
-
 polyP208
-
 Saccharomyces cerevisiae
3.6.1.11 0.093
-
 polyP15
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.11 vacuole
-
 Saccharomyces cerevisiae 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Co2+ stimulated by divalent cations, Co2+ is the best stimulator, 6fold at 0.05 mM Saccharomyces cerevisiae
3.6.1.11 Mg2+ stimulates Saccharomyces cerevisiae
3.6.1.11 Zn2+ stimulates Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 55000
-
 4 * 55000, SDS-PAGE Saccharomyces cerevisiae
3.6.1.11 245000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.11 60
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O the activity with polyP15 is 85% of that with polyP208, the activity with polyP9 is 24% of that with polyP208 Saccharomyces cerevisiae (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 adenosine 5'-tetraphosphate + H2O 7.3% of the activity with polyP208 Saccharomyces cerevisiae ATP + phosphate
-
 ?
3.6.1.11 polyP15 + H2O
-
 Saccharomyces cerevisiae polyP14 + phosphate
-
 ?
3.6.1.11 polyP208 + H2O
-
 Saccharomyces cerevisiae polyP207 + phosphate
-
 ?
3.6.1.11 tripolyphosphate + H2O 7.3% of the activity with polyP208 Saccharomyces cerevisiae phosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 tetramer 4 * 55000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 7
-
 about Saccharomyces cerevisiae