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Literature summary extracted from

  • Akiyama, M.; Crooke, E.; Kornberg, A.
    An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon (1993), J. Biol. Chem., 268, 633-639.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.11 (NH4)2SO4 50 mM, stimulates Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.11
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 phosphate
-
 Escherichia coli
3.6.1.11 Polyphosphate polyP15, 50% inhibition at 200fold polymer molar excess over the long-chain polyphosphate substrate Escherichia coli
3.6.1.11 Tetrapolyphosphate 50% inhibition at 2000fold polymer molar excess over the long-chain polyP substrate Escherichia coli
3.6.1.11 tripolyphosphate weak inhibition at 2000fold polymer molar excess over the long-chain polyP substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 KCl 175 mM, stimulates Escherichia coli
3.6.1.11 Mg2+ 1 mM, required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 57000
-
 2 * 57000, SDS-PAGE Escherichia coli
3.6.1.11 58133
-
 2 * 58133, calculation from nucleotide sequence Escherichia coli
3.6.1.11 100000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Escherichia coli
-
 K 12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O n = 500 Escherichia coli (polyphosphate)n-1 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 dimer 2 * 57000, SDS-PAGE Escherichia coli
3.6.1.11 dimer 2 * 58133, calculation from nucleotide sequence Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.11 37
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 8
-
 about Escherichia coli