EC Number | General Stability | Organism |
---|---|---|
3.6.1.1 | decreasing activity at 4°C in reaction mixture lacking diphosphate , KCl prevents from inactivation, Mg-diphosphate, does not protect from inactivation | Methanothermobacter thermautotrophicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | KF | 50% at 0.9 mM | Methanothermobacter thermautotrophicus | |
3.6.1.1 | Mn2+ | 60% inhibition at 1 mM Mn2+: 24 mM Mg2+ | Methanothermobacter thermautotrophicus | |
3.6.1.1 | NaF | - |
Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.1 | 0.16 | - |
diphosphate | - |
Methanothermobacter thermautotrophicus | |
3.6.1.1 | 4.9 | - |
Mg2+ | - |
Methanothermobacter thermautotrophicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.1 | cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | Co2+ | can partially replace Mg2+, 15% | Methanothermobacter thermautotrophicus | |
3.6.1.1 | Mg2+ | required for activity | Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 25000 | - |
4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
3.6.1.1 | 100000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | Methanothermobacter thermautotrophicus | - |
2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | Methanothermobacter thermautotrophicus H | - |
2 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
3.6.1.1 | Methanothermobacter thermautotrophicus H | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.1 | - |
Methanothermobacter thermautotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 1233 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Storage Stability | Organism |
---|---|---|
3.6.1.1 | -20°C, Tris-HCl, one year, negligible loss of activity | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | - |
Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.1 | tetramer | 4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 70 | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 7.7 | - |
60°C | Methanothermobacter thermautotrophicus |