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Literature summary extracted from

  • van Alebeek, G.J.W.; Keltjens, J.T.; van der Drift, C.
    Purification and characterization of inorganic pyrophosphatase from Methanobacterium thermoautotrophicum (strain H) (1994), Biochim. Biophys. Acta, 1206, 231-239.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.1 decreasing activity at 4°C in reaction mixture lacking diphosphate , KCl prevents from inactivation, Mg-diphosphate, does not protect from inactivation Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 KF 50% at 0.9 mM Methanothermobacter thermautotrophicus
3.6.1.1 Mn2+ 60% inhibition at 1 mM Mn2+: 24 mM Mg2+ Methanothermobacter thermautotrophicus
3.6.1.1 NaF
-
Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.16
-
diphosphate
-
Methanothermobacter thermautotrophicus
3.6.1.1 4.9
-
Mg2+
-
Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytosol
-
Methanothermobacter thermautotrophicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ can partially replace Mg2+, 15% Methanothermobacter thermautotrophicus
3.6.1.1 Mg2+ required for activity Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 25000
-
4 * 25000, gel filtration, dimeric form also active Methanothermobacter thermautotrophicus
3.6.1.1 100000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Methanothermobacter thermautotrophicus
-
2 phosphate
-
?
3.6.1.1 diphosphate + H2O Methanothermobacter thermautotrophicus H
-
2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Methanothermobacter thermautotrophicus
-
-
-
3.6.1.1 Methanothermobacter thermautotrophicus H
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 1233
-
-
Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
3.6.1.1 -20°C, Tris-HCl, one year, negligible loss of activity Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
Methanothermobacter thermautotrophicus 2 phosphate
-
?
3.6.1.1 diphosphate + H2O high specificity Methanothermobacter thermautotrophicus 2 phosphate
-
?
3.6.1.1 diphosphate + H2O
-
Methanothermobacter thermautotrophicus H 2 phosphate
-
?
3.6.1.1 diphosphate + H2O high specificity Methanothermobacter thermautotrophicus H 2 phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 tetramer 4 * 25000, gel filtration, dimeric form also active Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 70
-
-
Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 7.7
-
60°C Methanothermobacter thermautotrophicus