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Literature summary extracted from

  • Jiang, S.S.; Fan, L.L.; Yang, S.J.; Kuo, S.Y.; Pan, R.L.
    Purification and characterization of thylakoid membrane-bound inorganic pyrophosphatase from Spinacia oleracea L. (1997), Arch. Biochem. Biophys., 346, 105-112.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 Ca2+ less effective than Cd2+ Spinacia oleracea
3.6.1.1 Cd2+
-
 Spinacia oleracea
3.6.1.1 Co2+ less effective than Ca2+ Spinacia oleracea
3.6.1.1 Cu2+ less effective then Mn2+ Spinacia oleracea
3.6.1.1 diphosphate above 1 mM Spinacia oleracea
3.6.1.1 F-
-
 Spinacia oleracea
3.6.1.1 Mn2+ less effective than Zn2+ Spinacia oleracea
3.6.1.1 Zn2+ less effective then Co2+ Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 membrane membrane bound Spinacia oleracea 16020
-
3.6.1.1 thylakoid
-
 Spinacia oleracea 9579
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mg2+ required for activity Spinacia oleracea
3.6.1.1 Mg2+ no other divalent cations can substitute for Mg2+ Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 55000
-
 gel filtration, SDS-PAGE, amino acid sequence Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Spinacia oleracea actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 6.7
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Spinacia oleracea 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O high specificity Spinacia oleracea 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 monomer 1 * 55000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 8.5 9
-
 Spinacia oleracea