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Literature summary extracted from
- Properties of apyrase and inorganic pyrophosphatase in Streptomyces aureofaciens (1982), Folia Microbiol. (Praha), 27, 159-166.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Ca2+ | - |
Kitasatospora aureofaciens |  |
| 3.6.1.1 | Co2+ | - |
Kitasatospora aureofaciens | |
| 3.6.1.1 | EDTA | - |
Kitasatospora aureofaciens | |
| 3.6.1.5 | Ca2+ | - |
Kitasatospora aureofaciens | |
| 3.6.1.5 | Co2+ | - |
Kitasatospora aureofaciens | |
| 3.6.1.5 | dinitrophenol | - |
Kitasatospora aureofaciens | |
| 3.6.1.5 | iodoacetic acid | - |
Kitasatospora aureofaciens | |
| 3.6.1.5 | oligomycin | - |
Kitasatospora aureofaciens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.1 | membrane | - |
Kitasatospora aureofaciens | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Co2+ | about 20% relative activity to Mg2+ when above 3 mM | Kitasatospora aureofaciens | |
| 3.6.1.1 | Mg2+ | maximum activity at 3 mM | Kitasatospora aureofaciens | |
| 3.6.1.5 | Mg2+ | required for activity | Kitasatospora aureofaciens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | diphosphate + H2O | Kitasatospora aureofaciens | - |
2 phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | Kitasatospora aureofaciens RIA 57 | - |
2 phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | Kitasatospora aureofaciens RIA57 | - |
2 phosphate | - |
? | |
| 3.6.1.5 | ADP + H2O | Kitasatospora aureofaciens | - |
AMP + phosphate | - |
? | |
| 3.6.1.5 | ATP + 2 H2O | Kitasatospora aureofaciens | - |
AMP + 2 phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.1 | Kitasatospora aureofaciens | - |
- |
- |
| 3.6.1.1 | Kitasatospora aureofaciens RIA57 | - |
- |
- |
| 3.6.1.5 | Kitasatospora aureofaciens | - |
- |
- |
| 3.6.1.5 | Kitasatospora aureofaciens RIA 57 | - |
- |
- |
| 3.6.1.11 | Kitasatospora aureofaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.5 | partially | Kitasatospora aureofaciens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.1 | mycelium | - |
Kitasatospora aureofaciens | - |
| 3.6.1.5 | mycelium | - |
Kitasatospora aureofaciens | - |
| 3.6.1.11 | mycelium | - |
Kitasatospora aureofaciens | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.6.1.5 | 0-4°C, 25 mM Tris.HCl, pH 7.4, 7 days, no loss of activity | Kitasatospora aureofaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | ADP + H2O | 18% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | ADP + H2O | 18% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA 57 | ? | - |
? | |
| 3.6.1.1 | ADP + H2O | 18% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA57 | ? | - |
? | |
| 3.6.1.1 | AMP + H2O | 3.6% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | 7.8% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | 7.8% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA 57 | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | 7.8% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA57 | ? | - |
? | |
| 3.6.1.1 | D-fructose-6-phosphate + H2O | 13% relative activity compared to diphosphate | Kitasatospora aureofaciens | D-fructose + phosphate | - |
? | |
| 3.6.1.1 | D-glucose-1-phosphate + H2O | 15% relative activity compared to diphosphate | Kitasatospora aureofaciens | D-glucose + phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | - |
Kitasatospora aureofaciens | 2 phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | - |
Kitasatospora aureofaciens RIA 57 | 2 phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | - |
Kitasatospora aureofaciens RIA57 | 2 phosphate | - |
? | |
| 3.6.1.1 | GDP + H2O | 20% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | GMP + H2O | 18% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | phosphoenolpyruvate + H2O | 12% relative activity compared to diphosphate | Kitasatospora aureofaciens | pyruvate + phosphate | - |
? | |
| 3.6.1.1 | phosphoenolpyruvate + H2O | 12% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA 57 | pyruvate + phosphate | - |
? | |
| 3.6.1.1 | phosphoenolpyruvate + H2O | 12% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA57 | pyruvate + phosphate | - |
? | |
| 3.6.1.1 | UTP + H2O | 32% relative activity compared to diphosphate | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.1 | UTP + H2O | 32% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA 57 | ? | - |
? | |
| 3.6.1.1 | UTP + H2O | 32% relative activity compared to diphosphate | Kitasatospora aureofaciens RIA57 | ? | - |
? | |
| 3.6.1.5 | ADP + H2O | - |
Kitasatospora aureofaciens | AMP + phosphate | - |
? | |
| 3.6.1.5 | ATP + 2 H2O | - |
Kitasatospora aureofaciens | AMP + 2 phosphate | - |
? | |
| 3.6.1.5 | CDP + H2O | - |
Kitasatospora aureofaciens | CMP + phosphate | - |
? | |
| 3.6.1.5 | CTP + 2 H2O | - |
Kitasatospora aureofaciens | CMP + 2 phosphate | - |
? | |
| 3.6.1.5 | GDP + H2O | - |
Kitasatospora aureofaciens | GMP + phosphate | - |
? | |
| 3.6.1.5 | GTP + 2 H2O | - |
Kitasatospora aureofaciens | GMP + 2 phosphate | - |
? | |
| 3.6.1.5 | IDP + H2O | - |
Kitasatospora aureofaciens | IMP + phosphate | - |
? | |
| 3.6.1.5 | ITP + 2 H2O | - |
Kitasatospora aureofaciens | IMP + 2 phosphate | - |
? | |
| 3.6.1.5 | additional information | neglible hydrolysis of AMP | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.5 | oligophosphates + H2O | n = 3, 15, 40, 60 | Kitasatospora aureofaciens | ? | - |
? | |
| 3.6.1.5 | phosphoenolpyruvate + H2O | - |
Kitasatospora aureofaciens | pyruvate + phosphate | - |
? | |
| 3.6.1.5 | thiamine diphosphate + 2 H2O | - |
Kitasatospora aureofaciens | thiamine + 2 phosphate | - |
? | |
| 3.6.1.5 | UDP + H2O | - |
Kitasatospora aureofaciens | UMP + phosphate | - |
? | |
| 3.6.1.5 | UTP + 2 H2O | - |
Kitasatospora aureofaciens | UMP + 2 phosphate | - |
? | |
| 3.6.1.11 | (polyphosphate)n + H2O | - |
Kitasatospora aureofaciens | (polyphosphate)n-1 + phosphate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 35 | 40 | - |
Kitasatospora aureofaciens |
| 3.6.1.5 | 35 | 40 | - |
Kitasatospora aureofaciens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 20 | 50 | - |
Kitasatospora aureofaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 7.4 | - |
- |
Kitasatospora aureofaciens |
| 3.6.1.5 | 7.4 | - |
- |
Kitasatospora aureofaciens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 6 | 9 | - |
Kitasatospora aureofaciens |
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