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Literature summary extracted from

  • Schwarm, H.M.; Vigenschow, H.; Knobloch, K.
    Purification and properties of a soluble inorganic pyrophosphatase from Rhodopseudomonas palustris (1986), Biol. Chem. Hoppe-Seyler, 367, 119-126.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
 Rhodopseudomonas palustris
3.6.1.1 Ba2+
-
 Rhodopseudomonas palustris
3.6.1.1 EDTA
-
 Rhodopseudomonas palustris
3.6.1.1 F-
-
 Rhodopseudomonas palustris
3.6.1.1 free divalent cations
-
 Rhodopseudomonas palustris
3.6.1.1 I2
-
 Rhodopseudomonas palustris
3.6.1.1 Imidodiphosphate
-
 Rhodopseudomonas palustris
3.6.1.1 iodoacetate
-
 Rhodopseudomonas palustris
3.6.1.1 phenylmercuric acetate
-
 Rhodopseudomonas palustris
3.6.1.1 Zn2+
-
 Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.19
-
 Ca-diphosphate
-
 Rhodopseudomonas palustris
3.6.1.1 0.23
-
 Mg-diphosphate
-
 Rhodopseudomonas palustris
3.6.1.1 0.29
-
 Co-diphosphate
-
 Rhodopseudomonas palustris
3.6.1.1 0.37
-
 Mn-diphosphate
-
 Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Ca2+ most potent activator Rhodopseudomonas palustris
3.6.1.1 Co2+ activating Rhodopseudomonas palustris
3.6.1.1 Mg2+
-
 Rhodopseudomonas palustris
3.6.1.1 Mn2+ activating Rhodopseudomonas palustris
3.6.1.1 Zn2+ activating Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 32000
-
 2 * 32000, SDS-PAGE Rhodopseudomonas palustris
3.6.1.1 64500
-
-
 Rhodopseudomonas palustris
3.6.1.1 65500
-
 gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Rhodopseudomonas palustris actual substrate is magnesium diphosphate or dimagnesium diphosphate 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
3.6.1.1 -20°C, for several months Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O actual substrate is magnesium diphosphate or dimagnesium diphosphate Rhodopseudomonas palustris 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O diphosphate in form of Mg-diphosphate Rhodopseudomonas palustris 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O diphosphate in form of Ca-diphosphate Rhodopseudomonas palustris 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O diphosphate in form of Co-diphosphate Rhodopseudomonas palustris 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O diphosphate in form of Mn-diphosphate Rhodopseudomonas palustris 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 dimer 2 * 32000, SDS-PAGE Rhodopseudomonas palustris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 60
-
 rapid inactivation Rhodopseudomonas palustris