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Literature summary extracted from

  • Wang, Y.; Leigh, R.A.; Kaestner, K.H.; Sze, H.
    Electrogenic H+-pumping pyrophosphatase in tonoplast vesicles of oat roots (1986), Plant Physiol., 81, 497-502.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.3.1 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
 Avena sativa
7.1.3.1 F- IC50: 1-2 mM Avena sativa
7.1.3.1 Li+
-
 Avena sativa
7.1.3.1 N-ethylmaleimide
-
 Avena sativa
7.1.3.1 Na+
-
 Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.3.1 0.015
-
 diphosphate
-
 Avena sativa
7.1.3.1 4
-
 K+
-
 Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.3.1 tonoplast
-
 Avena sativa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.3.1 K+ required for activity Avena sativa
7.1.3.1 Mg2+
-
 Avena sativa
7.1.3.1 Rb+
-
 Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1] Avena sativa H+-pump 2 phosphate + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.3.1 Avena sativa
-
 oat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.3.1 root
-
 Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Avena sativa 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] H+-pump Avena sativa 2 phosphate + H+[side 2]
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.1.3.1 4
-
 3-4 h, 50% loss of activity Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.3.1 8
-
-
 Avena sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.1.3.1 1 2 IC50: 1-2 mM Avena sativa F-