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Literature summary extracted from

  • Krishnan, V.A.; Gnanam, A.
    Properties and regulation of Mg2+-dependent chloroplast inorganic pyrophosphatase from Sorghum vulgare leaves (1988), Arch. Biochem. Biophys., 260, 277-284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 Ca2+
-
 Sorghum bicolor
3.6.1.1 Cd2+
-
 Sorghum bicolor
3.6.1.1 Co2+
-
 Sorghum bicolor
3.6.1.1 EDTA
-
 Sorghum bicolor
3.6.1.1 L-malate allosteric mechanism Sorghum bicolor
3.6.1.1 Mn2+
-
 Sorghum bicolor
3.6.1.1 Zn2+
-
 Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.035
-
 diphosphate
-
 Sorghum bicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 chloroplast from mesophyll Sorghum bicolor 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mg2+ no other divalent cations can substitute for Mg2+ Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 42000
-
 gel filtration, SDS-PAGE Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Sorghum bicolor
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Sorghum bicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1
-
 Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 leaf
-
 Sorghum bicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 478
-
-
 Sorghum bicolor

Storage Stability

EC Number Storage Stability Organism
3.6.1.1 0-4°C, (NH4)2SO4, 65% saturated, a few months, activity retained Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 D-glucose-1-phosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor D-glucose + phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Sorghum bicolor 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O high specificity Sorghum bicolor 2 phosphate
-
 ?
3.6.1.1 tetrapolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ?
-
 ?
3.6.1.1 tripolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 monomer 1 * 42000 Sorghum bicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1
-
 25 stable to freezing, 0°C and thawing 25° Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 8
-
-
 Sorghum bicolor