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Literature summary extracted from
- A thiocyanate hydrolase of Thiobacillus thioparus: a novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate (1992), J. Biol. Chem., 267, 9170-9175.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.8 | 11 | - |
thiocyanate | - |
Thiobacillus thioparus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 19000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
| 3.5.5.8 | 23000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
| 3.5.5.8 | 32000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
| 3.5.5.8 | 126000 | - |
gel filtration | Thiobacillus thioparus |
| 3.5.5.8 | 142000 | - |
native PAGE | Thiobacillus thioparus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.8 | thiocyanate + H2O | Thiobacillus thioparus | - |
carbonyl sulfide + NH3 + OH- | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.8 | Thiobacillus thioparus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.8 | ammonium sulfate precipitation, DEAE-Sephacyl, hydroxylapatite | Thiobacillus thioparus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 23.3 | - |
- |
Thiobacillus thioparus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.8 | thiocyanate + H2O | - |
Thiobacillus thioparus | carbonyl sulfide + NH3 + OH- | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.8 | hexamer | 2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 35 | - |
- |
Thiobacillus thioparus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 30 | 40 | enzyme shows high activity within this range | Thiobacillus thioparus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 60 | - |
treatment of the enzyme at 60°C for 5 min decreases activity below 10% of the original | Thiobacillus thioparus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.8 | 7.5 | 8 | - |
Thiobacillus thioparus |
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