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Literature summary extracted from

  • Thimann, K.W.; Mahadevan, S.
    Nitrilase. I. Occurrence, preparation, and general properties of the enzyme (1964), Arch. Biochem. Biophys., 105, 133-141.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 4-hydroxymercuribenzoic acid complete inhibition at 0.1 mM, reversal by GSH Hordeum vulgare
3.5.5.1 Ag+ complete inhibition between 0.1 and 1 mM Hordeum vulgare
3.5.5.1 Cu2+ complete inhibition at 1 mM Hordeum vulgare
3.5.5.1 Hg2+ complete inhibition between 0.1 and 1 mM Hordeum vulgare
3.5.5.1 N-ethylmaleimide 30% inhibition at 1 mM Hordeum vulgare
3.5.5.1 Zn2+ complete inhibition at 1 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 0.054
-
 indole-3-acetonitrile
-
 Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.5.1 cytoplasm
-
 Hordeum vulgare 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 indol-3-acetonitrile + H2O Hordeum vulgare
-
 indol-3-acetic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Hordeum vulgare
-
-
-
3.5.5.1 no activity in Allium cepa
-
-
-
3.5.5.1 no activity in Citrus limon
-
-
-
3.5.5.1 no activity in Croton sp.
-
-
-
3.5.5.1 no activity in Cucumis sativus
-
-
-
3.5.5.1 no activity in Ginkgo biloba
-
-
-
3.5.5.1 no activity in Helianthus annuus
-
-
-
3.5.5.1 no activity in Heliconia sp.
-
-
-
3.5.5.1 no activity in Impatiens balsamina
-
-
-
3.5.5.1 no activity in Iris sp.
-
-
-
3.5.5.1 no activity in Kalanchoe blossfeldiana
-
-
-
3.5.5.1 no activity in Lilium sp.
-
-
-
3.5.5.1 no activity in Monstera deliciosa
-
-
-
3.5.5.1 no activity in Nerium oleander
-
-
-
3.5.5.1 no activity in Onoclea sensibilis
-
-
-
3.5.5.1 no activity in Pelargonium zonale
-
-
-
3.5.5.1 no activity in Pteridium aquilinum
-
-
-
3.5.5.1 no activity in Rosa sp.
-
-
-
3.5.5.1 no activity in Solanum lycopersicum
-
-
-
3.5.5.1 no activity in Tradescantia virginiana
-
-
-
3.5.5.1 no activity in Xanthium pennsylvanicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity, 2step ammonium phosphate precipitation, calcium phosphate gel Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 leaf
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 indol-3-acetonitrile + H2O
-
 Hordeum vulgare indol-3-acetic acid + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 35
-
 decreasing activity above Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7.1
-
 at 35°C Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 5.5 8 at 25°C Hordeum vulgare