BRENDA - Enzyme Database

Asymmetric hydrolysis of alpha-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34

Bhalla, T.C.; Miura, A.; Wakamoto, A.; Ohba, Y.; Furuhashi, K.; Appl. Microbiol. Biotechnol. 37, 184-190 (1992)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.5.1
hydroxylamine
20% activation at 1 mM
Rhodococcus rhodochrous
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.5.1
4-chloromercuribenzoate
nearly complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
5,5'-dithiobis(2-nitrobenzoic acid)
complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
Ag+
complete inhibition at 5 mM
Rhodococcus rhodochrous
3.5.5.1
Co2+
complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
Hg2+
43% inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
iodoacetic acid
slight inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
o-phenanthroline
complete inhibition at 5 mM
Rhodococcus rhodochrous
3.5.5.1
Zn2+
complete inhibition at 1 mM
Rhodococcus rhodochrous
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.5.1
45000
-
gel filtration
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.5.1
acrylonitrile + H2O
Rhodococcus rhodochrous
-
acrylic acid + NH3
-
Rhodococcus rhodochrous
?
3.5.5.1
benzonitrile + H2O
Rhodococcus rhodochrous
best substrate
benzoic acid + NH3
-
Rhodococcus rhodochrous
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.5.1
Rhodococcus rhodochrous
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.5.1
to homogeneity, chromatography techniques
Rhodococcus rhodochrous
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.5.1
cell culture
best inducer of enzyme formation: isobutyronitrile
Rhodococcus rhodochrous
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.5.1
3.52
-
-
Rhodococcus rhodochrous
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.5.1
2-aminobutyronitrile + H2O
-
209755
Rhodococcus rhodochrous
2-aminobutyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
2-aminopropionitrile + H2O
low activity
209755
Rhodococcus rhodochrous
2-aminopropionic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
2-methylbutyronitrile + H2O
-
209755
Rhodococcus rhodochrous
2-methylbutyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
acrylonitrile + H2O
-
209755
Rhodococcus rhodochrous
acrylic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
benzonitrile + H2O
best substrate
209755
Rhodococcus rhodochrous
benzoic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
crotononitrile + H2O
-
209755
Rhodococcus rhodochrous
NH3 + (z)-but-2-en-1-ol
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-4-methylthio butyronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-methionine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-n-capronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-norleucine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-n-valeronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-norvaline + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminoisocapronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-leucine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminoisovaleronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-valine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminopropionitrile + H2O
-
209755
Rhodococcus rhodochrous
D-alanine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
lactonitrile + H2O
-
209755
Rhodococcus rhodochrous
lactate + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
mandelonitrile + 2 H2O
-
209755
Rhodococcus rhodochrous
mandelic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
methacrylonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
methylacrylic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
n-butyronitrile + H2O
-
209755
Rhodococcus rhodochrous
n-butyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
phenylacetonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
phenylacetate + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
phenylglycinonitrile + H2O
low actiity
209755
Rhodococcus rhodochrous
phenylglycine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
propionitrile + H2O
-
209755
Rhodococcus rhodochrous
propionic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
succinonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
succinate + NH3
-
209755
Rhodococcus rhodochrous
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.5.1
monomer
1 * 45000, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.5.1
35
-
-
Rhodococcus rhodochrous
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.5.1
35
-
decreasing activity above
Rhodococcus rhodochrous
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.5.1
7.5
-
-
Rhodococcus rhodochrous
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.5.1
hydroxylamine
20% activation at 1 mM
Rhodococcus rhodochrous
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.5.1
4-chloromercuribenzoate
nearly complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
5,5'-dithiobis(2-nitrobenzoic acid)
complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
Ag+
complete inhibition at 5 mM
Rhodococcus rhodochrous
3.5.5.1
Co2+
complete inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
Hg2+
43% inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
iodoacetic acid
slight inhibition at 1 mM
Rhodococcus rhodochrous
3.5.5.1
o-phenanthroline
complete inhibition at 5 mM
Rhodococcus rhodochrous
3.5.5.1
Zn2+
complete inhibition at 1 mM
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.5.1
45000
-
gel filtration
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.5.1
acrylonitrile + H2O
Rhodococcus rhodochrous
-
acrylic acid + NH3
-
Rhodococcus rhodochrous
?
3.5.5.1
benzonitrile + H2O
Rhodococcus rhodochrous
best substrate
benzoic acid + NH3
-
Rhodococcus rhodochrous
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.5.1
to homogeneity, chromatography techniques
Rhodococcus rhodochrous
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.5.1
cell culture
best inducer of enzyme formation: isobutyronitrile
Rhodococcus rhodochrous
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.5.1
3.52
-
-
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.5.1
2-aminobutyronitrile + H2O
-
209755
Rhodococcus rhodochrous
2-aminobutyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
2-aminopropionitrile + H2O
low activity
209755
Rhodococcus rhodochrous
2-aminopropionic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
2-methylbutyronitrile + H2O
-
209755
Rhodococcus rhodochrous
2-methylbutyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
acrylonitrile + H2O
-
209755
Rhodococcus rhodochrous
acrylic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
benzonitrile + H2O
best substrate
209755
Rhodococcus rhodochrous
benzoic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
crotononitrile + H2O
-
209755
Rhodococcus rhodochrous
NH3 + (z)-but-2-en-1-ol
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-4-methylthio butyronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-methionine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-n-capronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-norleucine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-amino-n-valeronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-norvaline + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminoisocapronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-leucine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminoisovaleronitrile + H2O
-
209755
Rhodococcus rhodochrous
L-valine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
DL-alpha-aminopropionitrile + H2O
-
209755
Rhodococcus rhodochrous
D-alanine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
lactonitrile + H2O
-
209755
Rhodococcus rhodochrous
lactate + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
mandelonitrile + 2 H2O
-
209755
Rhodococcus rhodochrous
mandelic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
methacrylonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
methylacrylic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
n-butyronitrile + H2O
-
209755
Rhodococcus rhodochrous
n-butyric acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
phenylacetonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
phenylacetate + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
phenylglycinonitrile + H2O
low actiity
209755
Rhodococcus rhodochrous
phenylglycine + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
propionitrile + H2O
-
209755
Rhodococcus rhodochrous
propionic acid + NH3
-
209755
Rhodococcus rhodochrous
?
3.5.5.1
succinonitrile + H2O
low activity
209755
Rhodococcus rhodochrous
succinate + NH3
-
209755
Rhodococcus rhodochrous
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.5.1
monomer
1 * 45000, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.5.1
35
-
-
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.5.1
35
-
decreasing activity above
Rhodococcus rhodochrous
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.5.1
7.5
-
-
Rhodococcus rhodochrous