Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bartling, D.; Seedorf, M.; Schmidt, R.C.; Weiler, E.W.
    Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: Key enzymes in biosynthesis of the plant hormone indole-3-acetic acid (1994), Proc. Natl. Acad. Sci. USA, 91, 6021-6025.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.5.1 cytoplasm nitrilase I Arabidopsis thaliana 5737
-
3.5.5.1 microsome nitrilase II Arabidopsis thaliana
-
-
3.5.5.1 plasma membrane nitrilase II, tightly associated Arabidopsis thaliana 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 indol-3-acetonitrile + H2O Arabidopsis thaliana
-
 indol-3-acetic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 nitrilase II, to homogeneity, recombinant enzyme Arabidopsis thaliana

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.5.1 release from insoluble inclusion bodies after treatment with Triton X-114 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 whole plant 2 forms, nitrilase I and II Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 indol-3-acetonitrile + H2O
-
 Arabidopsis thaliana indol-3-acetic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 nirilase II
-
 Arabidopsis thaliana
3.5.5.1 nitrilase I
-
 Arabidopsis thaliana