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Literature summary extracted from

  • Pomper, B.K.; Vorholt, J.A.; Chistoserdova, L.; Lidstrom, M.E.; Thauer, R.K.
    A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1 (1999), Eur. J. Biochem., 261, 475-480.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.9 0.08
-
 5,10-methenyltetrahydrofolate
-
 Methylorubrum extorquens
3.5.4.27 0.03
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methylorubrum extorquens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.27 NaCl presence of salts required, activity in absence of salts is only 15% of that in the presence of salts Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.9 22000
-
 2 * 22000, SDS-PAGE Methylorubrum extorquens
3.5.4.9 45000
-
 gel filtration Methylorubrum extorquens
3.5.4.27 33000
-
 2 * 33000, SDS-PAGE Methylorubrum extorquens
3.5.4.27 67000
-
 non-denaturing PAGE Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Methylorubrum extorquens
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methylorubrum extorquens the enzyme might have a catabolic function in the C1-unit metabolism ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 the enzyme might have a catabolic function in the C1-unit metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Methylorubrum extorquens
-
 AM1, aerobic methylotrophic proteobacterium, monofunctional enzyme
-
3.5.4.27 Methylorubrum extorquens
-
-
-
3.5.4.27 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.9
-
 Methylorubrum extorquens
3.5.4.27
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 104
-
-
 Methylorubrum extorquens
3.5.4.27 104
-
-
 Methylorubrum extorquens
3.5.4.27 630
-
-
 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Methylorubrum extorquens 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 10-formyltetrahydrofolate
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methylorubrum extorquens N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O the enzyme might have a catabolic function in the C1-unit metabolism Methylorubrum extorquens ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O the enzyme might have a catabolic function in the C1-unit metabolism Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.9 dimer 2 * 22000, SDS-PAGE Methylorubrum extorquens
3.5.4.27 dimer 2 * 33000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.9 methenylH4F cyclohydrolase
-
 Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.27 40
-
-
 Methylorubrum extorquens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.27 additional information
-
 salts increase heat stability of the enzyme Methylorubrum extorquens
3.5.4.27 60
-
 10 min, 50% loss of activity in absence of salts. Stable for 25 min in presence of salt, 1 M K2HPO4 Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.27 8.5
-
-
 Methylorubrum extorquens