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Literature summary extracted from
- Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli (1991), J. Biol. Chem., 266, 23953-23958.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.9 | gene folD for bifunctional dehydrogenase/cyclohydrolase cloned and sequenced | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.4.9 | cytoplasm | - |
Mus musculus | 5737 | - |
| 3.5.4.9 | cytoplasm | - |
Homo sapiens | 5737 | - |
| 3.5.4.9 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
| 3.5.4.9 | cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| 3.5.4.9 | cytoplasm | - |
Columba sp. | 5737 | - |
| 3.5.4.9 | mitochondrion | - |
Mus musculus | 5739 | - |
| 3.5.4.9 | mitochondrion | - |
Homo sapiens | 5739 | - |
| 3.5.4.9 | mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.9 | 31060 | - |
calculated from amino acid sequence | Escherichia coli |
| 3.5.4.9 | 35000 | - |
SDS-PAGE | Escherichia coli |
| 3.5.4.9 | 70000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Gallus gallus | - |
10-formyltetrahydrofolate | - |
? |  |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Mus musculus | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Homo sapiens | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Rattus norvegicus | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Sus scrofa | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Oryctolagus cuniculus | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Ovis aries | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Clostridium cylindrosporum | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Columba sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | Escherichia coli BE | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.9 | Clostridium cylindrosporum | - |
- |
- |
| 3.5.4.9 | Columba sp. | - |
pigeon | - |
| 3.5.4.9 | Escherichia coli | - |
bifunctional enzyme | - |
| 3.5.4.9 | Escherichia coli BE | - |
bifunctional enzyme | - |
| 3.5.4.9 | Gallus gallus | - |
chicken, trifunctional enzyme | - |
| 3.5.4.9 | Homo sapiens | - |
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme | - |
| 3.5.4.9 | Moorella thermoacetica | - |
bifunctional enzyme | - |
| 3.5.4.9 | Mus musculus | - |
Ehrlich ascites cells | - |
| 3.5.4.9 | Mus musculus | - |
mouse, bifunctional mitochondrial enzyme | - |
| 3.5.4.9 | Oryctolagus cuniculus | - |
rabbit | - |
| 3.5.4.9 | Ovis aries | - |
sheep, trifunctional enzyme | - |
| 3.5.4.9 | Rattus norvegicus | - |
rat, trifunctional cytoplasmic enzyme | - |
| 3.5.4.9 | Saccharomyces cerevisiae | - |
yeast, trifunctional enzyme | - |
| 3.5.4.9 | Sus scrofa | - |
pig, trifunctional enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.9 | - |
Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.9 | liver | - |
Gallus gallus | - |
| 3.5.4.9 | liver | - |
Mus musculus | - |
| 3.5.4.9 | liver | - |
Rattus norvegicus | - |
| 3.5.4.9 | liver | - |
Sus scrofa | - |
| 3.5.4.9 | liver | - |
Oryctolagus cuniculus | - |
| 3.5.4.9 | liver | - |
Ovis aries | - |
| 3.5.4.9 | liver | - |
Columba sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.9 | 31 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.9 | -20°C purified enzyme in 50% glycerol, 95% active after 6 months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Gallus gallus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Mus musculus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Rattus norvegicus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Sus scrofa | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Oryctolagus cuniculus | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Ovis aries | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Clostridium cylindrosporum | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Columba sp. | 10-formyltetrahydrofolate | - |
? | |
| 3.5.4.9 | 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli BE | 10-formyltetrahydrofolate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.9 | dimer | - |
Saccharomyces cerevisiae |
| 3.5.4.9 | dimer | 2 * 35000, SDS-PAGE | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.9 | 8.5 | - |
bifunctional enzyme | Escherichia coli |
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