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Literature summary extracted from

  • Tabor, H.; Wyngarden, L.
    The enzymatic formation of formiminotetrahydrofolic acid, 5,10-methenyltetrahydrofolic acid, and 10-formyltetrahydrofolic acid in the metabolism of formiminoglutamic acid (1959), J. Biol. Chem., 234, 1830-1846.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.5 aminopterin 0.0005 mM, 53% inhibition Sus scrofa
2.1.2.5 barium acetate 0.1 M, 90% inhibition Sus scrofa
2.1.2.5 CaCl2 0.1 M, 72% inhibition Sus scrofa
2.1.2.5 KCl
-
 Bos taurus
2.1.2.5 KCl high concentrations Sus scrofa
2.1.2.5 methotrexate 0.0005 mM: 33% inhibition, 0.001 mM: 83% inhibition Sus scrofa
2.1.2.5 MnCl2 0.1 M: 98% inhibition, 0.01 M: 65% inhibition Sus scrofa
2.1.2.5 NH4Cl high concentrations Sus scrofa
2.1.2.5 ZnCl2 0.01 M, 99% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.5 0.1
-
 tetrahydrofolate
-
 Sus scrofa
2.1.2.5 11
-
 N-formimino-L-glutamate
-
 Sus scrofa
3.5.4.9 0.04
-
 5,10-methenyltetrahydrofolate
-
 Oryctolagus cuniculus
3.5.4.9 0.074
-
 5,10-methenyltetrahydrofolate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.5 KCl weak stimulation by low concentrations Sus scrofa
2.1.2.5 NH4+ NH4Cl: weak stimulation by low concentrations Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Cavia porcellus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Mus musculus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Homo sapiens histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Sus scrofa histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Bos taurus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Oryctolagus cuniculus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Felis catus histidine degradation 5-formiminotetrahydrofolate + L-glutamate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Cavia porcellus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Mus musculus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Rattus norvegicus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Sus scrofa
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Bos taurus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Oryctolagus cuniculus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Ovis aries
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Pseudomonas fluorescens
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Clostridium cylindrosporum
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Gottschalkia acidurici
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Felis sp.
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Bos taurus
-
-
-
2.1.2.5 Cavia porcellus
-
-
-
2.1.2.5 Felis catus
-
-
-
2.1.2.5 Homo sapiens
-
-
-
2.1.2.5 Mus musculus
-
-
-
2.1.2.5 Oryctolagus cuniculus
-
-
-
2.1.2.5 Sus scrofa
-
-
-
3.5.4.9 Bos taurus
-
 beef
-
3.5.4.9 Cavia porcellus
-
 guinea-pig
-
3.5.4.9 Clostridium cylindrosporum
-
-
-
3.5.4.9 Felis sp.
-
 cat
-
3.5.4.9 Gottschalkia acidurici
-
-
-
3.5.4.9 Homo sapiens
-
 human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme
-
3.5.4.9 Mus musculus
-
 mouse, bifunctional mitochondrial enzyme
-
3.5.4.9 Oryctolagus cuniculus
-
 rabbit
-
3.5.4.9 Ovis aries
-
-
-
3.5.4.9 Pseudomonas fluorescens
-
 ATCC 11299
-
3.5.4.9 Rattus norvegicus
-
 rat, trifunctional cytoplasmic enzyme
-
3.5.4.9 Sus scrofa
-
 pig, trifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.5 700fold purification Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 kidney low activity Cavia porcellus
-
2.1.2.5 kidney low activity Mus musculus
-
2.1.2.5 kidney low activity Sus scrofa
-
2.1.2.5 kidney low activity Bos taurus
-
2.1.2.5 kidney low activity Oryctolagus cuniculus
-
2.1.2.5 kidney low activity Felis catus
-
2.1.2.5 liver
-
 Cavia porcellus
-
2.1.2.5 liver
-
 Mus musculus
-
2.1.2.5 liver
-
 Homo sapiens
-
2.1.2.5 liver
-
 Sus scrofa
-
2.1.2.5 liver
-
 Bos taurus
-
2.1.2.5 liver
-
 Oryctolagus cuniculus
-
2.1.2.5 liver
-
 Felis catus
-
3.5.4.9 kidney
-
 Cavia porcellus
-
3.5.4.9 kidney
-
 Mus musculus
-
3.5.4.9 kidney
-
 Rattus norvegicus
-
3.5.4.9 kidney
-
 Sus scrofa
-
3.5.4.9 kidney
-
 Bos taurus
-
3.5.4.9 kidney
-
 Oryctolagus cuniculus
-
3.5.4.9 kidney
-
 Ovis aries
-
3.5.4.9 kidney
-
 Felis sp.
-
3.5.4.9 liver
-
 Cavia porcellus
-
3.5.4.9 liver
-
 Mus musculus
-
3.5.4.9 liver
-
 Rattus norvegicus
-
3.5.4.9 liver
-
 Sus scrofa
-
3.5.4.9 liver
-
 Bos taurus
-
3.5.4.9 liver
-
 Oryctolagus cuniculus
-
3.5.4.9 liver
-
 Ovis aries
-
3.5.4.9 liver
-
 Felis sp.
-
3.5.4.9 spleen
-
 Cavia porcellus
-
3.5.4.9 spleen
-
 Mus musculus
-
3.5.4.9 spleen
-
 Rattus norvegicus
-
3.5.4.9 spleen
-
 Oryctolagus cuniculus
-
3.5.4.9 spleen
-
 Felis sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.5 additional information
-
-
 Cavia porcellus
2.1.2.5 additional information
-
-
 Mus musculus
2.1.2.5 additional information
-
-
 Homo sapiens
2.1.2.5 additional information
-
-
 Sus scrofa
2.1.2.5 additional information
-
-
 Bos taurus
2.1.2.5 additional information
-
-
 Oryctolagus cuniculus
2.1.2.5 additional information
-
-
 Felis catus
3.5.4.9 0.09
-
 source spleen Oryctolagus cuniculus
3.5.4.9 0.1
-
 source spleen Cavia porcellus
3.5.4.9 0.1
-
 source kidney Ovis aries
3.5.4.9 0.13
-
 source kidney Bos taurus
3.5.4.9 0.14
-
-
 Homo sapiens
3.5.4.9 0.14
-
-
 Ovis aries
3.5.4.9 0.16
-
 source spleen Mus musculus
3.5.4.9 0.18
-
 source liver Rattus norvegicus
3.5.4.9 0.22
-
 source liver Sus scrofa
3.5.4.9 0.23
-
-
 Rattus norvegicus
3.5.4.9 0.23
-
 source spleen Felis sp.
3.5.4.9 0.25
-
 source kidney Oryctolagus cuniculus
3.5.4.9 0.27
-
 source liver Felis sp.
3.5.4.9 0.29
-
-
 Pseudomonas fluorescens
3.5.4.9 0.35
-
 source kidney Cavia porcellus
3.5.4.9 0.36
-
 source kidney Rattus norvegicus
3.5.4.9 0.4
-
 source liver Mus musculus
3.5.4.9 0.41
-
 source kidney Felis sp.
3.5.4.9 0.5
-
 source kidney Mus musculus
3.5.4.9 0.65
-
-
 Bos taurus
3.5.4.9 0.82
-
 source liver Oryctolagus cuniculus
3.5.4.9 1.04
-
 source liver Cavia porcellus
3.5.4.9 2.2
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.1.2.5 -20°C, several weeks, stable Sus scrofa
2.1.2.5 2°C, 2 months, 16-45% loss of activity Sus scrofa
2.1.2.5 2°C, several weeks, stable Sus scrofa
3.5.4.9 -20°C, loses 15% activity in 1 day, 50% in 5 days and 70% in 30 days Sus scrofa
3.5.4.9 0°C, relatively unstable, losing 40% activity in 1 day and 75% in 4 days Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 additional information primary reaction of the over-all reaction formimino-L-glutamate + tetrahydrofolate = N10-formyltetrahydrofolate + glutamate + NH3 Sus scrofa ?
-
 ?
2.1.2.5 additional information primary reaction of the over-all reaction formimino-L-glutamate + tetrahydrofolate = N10-formyltetrahydrofolate + glutamate + NH3 Bos taurus ?
-
 ?
2.1.2.5 additional information not: formiminoglycine, very poor substrates: formimino-L-aspartic acid, formyl-L-glutamic acid Sus scrofa ?
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Cavia porcellus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Mus musculus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Homo sapiens 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Sus scrofa 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Oryctolagus cuniculus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine degradation Felis catus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Cavia porcellus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Mus musculus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Homo sapiens 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Oryctolagus cuniculus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Felis catus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate relatively specific for formiminoglutamic acid Sus scrofa 5-formiminotetrahydrofolate + L-glutamate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Cavia porcellus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Mus musculus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Rattus norvegicus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Sus scrofa 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Bos taurus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Oryctolagus cuniculus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Ovis aries 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Pseudomonas fluorescens 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Clostridium cylindrosporum 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Gottschalkia acidurici 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Felis sp. 10-formyltetrahydrofolate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.5 25
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.5 7 8
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.5 6.2 9 about 50% of activity maximum at pH 6.2 and 9.0 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.5 tetrahydrofolate
-
 Cavia porcellus
2.1.2.5 tetrahydrofolate
-
 Mus musculus
2.1.2.5 tetrahydrofolate
-
 Homo sapiens
2.1.2.5 tetrahydrofolate
-
 Sus scrofa
2.1.2.5 tetrahydrofolate
-
 Bos taurus
2.1.2.5 tetrahydrofolate
-
 Oryctolagus cuniculus
2.1.2.5 tetrahydrofolate
-
 Felis catus