BRENDA - Enzyme Database show

A prokaryotic-type cytidine deaminase from Arabidopsis thaliana gene expression and functional characterization

Faivre-Nitschke, E.; Grienenberger, J.M.; Gualberto, J.M.; Eur. J. Biochem. 263, 896-903 (1999)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.4.5
medicine
-
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.5
gene At-cda1, located on chromosome 2 PCR amplified, cloned and sequenced, expressed in Escherichia coli BL21(D3)
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
blasticidin S
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Homo sapiens
3.5.4.5
CMP
-
Saccharomyces cerevisiae
3.5.4.5
dCMP
-
Arabidopsis thaliana
3.5.4.5
dCMP
-
Homo sapiens
3.5.4.5
NH3
-
Arabidopsis thaliana
3.5.4.5
NH3
-
Escherichia coli
3.5.4.5
uridine
-
Arabidopsis thaliana
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
uridine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.06
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.12
-
deoxycytidine
recombinant At-CDA1
Arabidopsis thaliana
3.5.4.5
0.22
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Arabidopsis thaliana
3.5.4.5
0.25
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.4.5
cytoplasm
cytosol
Arabidopsis thaliana
5737
-
3.5.4.5
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
31600
-
2 * 31600, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
32500
-
calculated from cDNA open reading frame
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Haemophilus influenzae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Arabidopsis thaliana
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Caenorhabditis elegans
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mycoplasma pneumoniae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Brugia pahangi
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli BL21(D3)
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Arabidopsis thaliana
-
ecotype Columbia
-
3.5.4.5
Bacillus subtilis
-
-
-
3.5.4.5
Brugia pahangi
-
-
-
3.5.4.5
Caenorhabditis elegans
-
-
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli BL21(D3)
-
-
-
3.5.4.5
Haemophilus influenzae
-
-
-
3.5.4.5
Homo sapiens
-
human
-
3.5.4.5
Mycoplasma pneumoniae
-
-
-
3.5.4.5
Saccharomyces cerevisiae
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, 50% glycerol, very stable, litle loss of enzymatic activity after 1 year
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2',3'-dideoxycytidine + H2O
-
209669
Homo sapiens
2',3'-dideoxyuridinel + NH3
-
-
-
?
3.5.4.5
2',3'-dideoxycytidine + H2O
selective inhibitor of HIV reverse transcriptase
209669
Arabidopsis thaliana
2',3'-dideoxyuridinel + NH3
-
-
-
-
3.5.4.5
5-azacytidine + H2O
-
209669
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Arabidopsis thaliana
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Escherichia coli BL21(D3)
5-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Haemophilus influenzae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Arabidopsis thaliana
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Caenorhabditis elegans
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Mycoplasma pneumoniae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Brugia pahangi
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Escherichia coli BL21(D3)
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Arabidopsis thaliana
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Escherichia coli BL21(D3)
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Arabidopsis thaliana
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Escherichia coli BL21(D3)
deoxyuridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 31600, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
tetramer
-
Homo sapiens
3.5.4.5
tetramer
-
Bacillus subtilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7.5
8
-
Arabidopsis thaliana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
6
11
-
Arabidopsis thaliana
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.4.5
medicine
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
gene At-cda1, located on chromosome 2 PCR amplified, cloned and sequenced, expressed in Escherichia coli BL21(D3)
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
blasticidin S
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Homo sapiens
3.5.4.5
CMP
-
Saccharomyces cerevisiae
3.5.4.5
dCMP
-
Arabidopsis thaliana
3.5.4.5
dCMP
-
Homo sapiens
3.5.4.5
NH3
-
Arabidopsis thaliana
3.5.4.5
NH3
-
Escherichia coli
3.5.4.5
uridine
-
Arabidopsis thaliana
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
uridine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.06
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.12
-
deoxycytidine
recombinant At-CDA1
Arabidopsis thaliana
3.5.4.5
0.22
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Arabidopsis thaliana
3.5.4.5
0.25
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.4.5
cytoplasm
cytosol
Arabidopsis thaliana
5737
-
3.5.4.5
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
31600
-
2 * 31600, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
32500
-
calculated from cDNA open reading frame
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Haemophilus influenzae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Arabidopsis thaliana
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Caenorhabditis elegans
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mycoplasma pneumoniae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Brugia pahangi
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli BL21(D3)
-
uridine + NH3
-
-
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, 50% glycerol, very stable, litle loss of enzymatic activity after 1 year
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2',3'-dideoxycytidine + H2O
-
209669
Homo sapiens
2',3'-dideoxyuridinel + NH3
-
-
-
?
3.5.4.5
2',3'-dideoxycytidine + H2O
selective inhibitor of HIV reverse transcriptase
209669
Arabidopsis thaliana
2',3'-dideoxyuridinel + NH3
-
-
-
-
3.5.4.5
5-azacytidine + H2O
-
209669
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Arabidopsis thaliana
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209669
Escherichia coli BL21(D3)
5-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Haemophilus influenzae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Arabidopsis thaliana
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Caenorhabditis elegans
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Mycoplasma pneumoniae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Brugia pahangi
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209669
Escherichia coli BL21(D3)
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Arabidopsis thaliana
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209669
Escherichia coli BL21(D3)
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Arabidopsis thaliana
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209669
Escherichia coli BL21(D3)
deoxyuridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 31600, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
tetramer
-
Homo sapiens
3.5.4.5
tetramer
-
Bacillus subtilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7.5
8
-
Arabidopsis thaliana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
6
11
-
Arabidopsis thaliana