BRENDA - Enzyme Database show

Cloning, expression, and purification of cytidine deaminase from Arabidopsis thaliana

Vincenzetti, S.; Cambi, A.; Neuhard, J.; Schnorr, K.; Grelloni, M.; Vita, A.; Protein Expr. Purif. 15, 8-15 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.5
cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201
Arabidopsis thaliana
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.4.5
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Arabidopsis thaliana
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Escherichia coli
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Mus musculus
3.5.4.5
3-deazauridine
-
Arabidopsis thaliana
3.5.4.5
5,6-Dihydrouridine
-
Arabidopsis thaliana
3.5.4.5
5,6-Dihydrouridine
-
Escherichia coli
3.5.4.5
5-fluoro-2'-deoxyuridine
-
Arabidopsis thaliana
3.5.4.5
5-fluorouridine
-
Arabidopsis thaliana
3.5.4.5
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Arabidopsis thaliana
3.5.4.5
thymine riboside
-
Arabidopsis thaliana
3.5.4.5
thymine riboside
-
Escherichia coli
3.5.4.5
uridine
-
Arabidopsis thaliana
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
UTP
-
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.058
-
cytosine arabinoside
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.06
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.075
-
deoxycytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.11
-
cytidine
-
Escherichia coli
3.5.4.5
0.15
-
cytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.22
-
cytosine arabinoside
-
Escherichia coli
3.5.4.5
0.31
-
5-azacytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.58
-
5-methylcytidine
recombinant
Arabidopsis thaliana
3.5.4.5
0.95
-
5-methylcytidine
-
Escherichia coli
3.5.4.5
2.27
-
5-azacytidine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
15000
-
4 * 15000
Gallus gallus
3.5.4.5
32000
-
2 * 32000, homodimer, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
32000
-
2 * 32000, homodimer, SDS-PAGE
Escherichia coli
3.5.4.5
32560
-
calculated from cDNA open reading frame
Arabidopsis thaliana
3.5.4.5
32580
-
calculated from amino acid sequence
Arabidopsis thaliana
3.5.4.5
63000
-
gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Gallus gallus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Haemophilus influenzae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Arabidopsis thaliana
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli SO5201
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Arabidopsis thaliana
-
-
-
3.5.4.5
Bacillus subtilis
-
-
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli SO5201
-
-
-
3.5.4.5
Gallus gallus
-
chicken
-
3.5.4.5
Haemophilus influenzae
-
-
-
3.5.4.5
Mus musculus
-
BALB/c; mouse
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
3.5.4.5
Ovis aries
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Arabidopsis thaliana
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
74
-
-
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-azacytidine + H2O
-
209668
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209668
Arabidopsis thaliana
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209668
Escherichia coli SO5201
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azadeoxycytidine + H2O
-
209668
Arabidopsis thaliana
5-azadeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Arabidopsis thaliana
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Escherichia coli SO5201
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Gallus gallus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Haemophilus influenzae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Arabidopsis thaliana
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Escherichia coli SO5201
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Arabidopsis thaliana
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Escherichia coli SO5201
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Arabidopsis thaliana
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Escherichia coli SO5201
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 32000, homodimer, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
dimer
2 * 32000, homodimer, SDS-PAGE
Escherichia coli
3.5.4.5
tetramer
4 * 15000
Gallus gallus
3.5.4.5
tetramer
-
Bacillus subtilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
3
10.5
deoxycytidine as substrate, specific activity constant in this range, beyond these values irreversible inactivation occurs
Arabidopsis thaliana
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
3
10.5
deoxycytidine as substrate, beyond these extremes rapid inactivation
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.4.5
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Arabidopsis thaliana
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Escherichia coli
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Mus musculus
3.5.4.5
3-deazauridine
-
Arabidopsis thaliana
3.5.4.5
5,6-Dihydrouridine
-
Arabidopsis thaliana
3.5.4.5
5,6-Dihydrouridine
-
Escherichia coli
3.5.4.5
5-fluoro-2'-deoxyuridine
-
Arabidopsis thaliana
3.5.4.5
5-fluorouridine
-
Arabidopsis thaliana
3.5.4.5
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine
-
Arabidopsis thaliana
3.5.4.5
CMP
-
Arabidopsis thaliana
3.5.4.5
thymine riboside
-
Arabidopsis thaliana
3.5.4.5
thymine riboside
-
Escherichia coli
3.5.4.5
uridine
-
Arabidopsis thaliana
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
UTP
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.058
-
cytosine arabinoside
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.06
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.075
-
deoxycytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.11
-
cytidine
-
Escherichia coli
3.5.4.5
0.15
-
cytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.22
-
cytosine arabinoside
-
Escherichia coli
3.5.4.5
0.31
-
5-azacytidine
recombinant AT-CDA
Arabidopsis thaliana
3.5.4.5
0.58
-
5-methylcytidine
recombinant
Arabidopsis thaliana
3.5.4.5
0.95
-
5-methylcytidine
-
Escherichia coli
3.5.4.5
2.27
-
5-azacytidine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
15000
-
4 * 15000
Gallus gallus
3.5.4.5
32000
-
2 * 32000, homodimer, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
32000
-
2 * 32000, homodimer, SDS-PAGE
Escherichia coli
3.5.4.5
32560
-
calculated from cDNA open reading frame
Arabidopsis thaliana
3.5.4.5
32580
-
calculated from amino acid sequence
Arabidopsis thaliana
3.5.4.5
63000
-
gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Gallus gallus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Haemophilus influenzae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Arabidopsis thaliana
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli SO5201
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Arabidopsis thaliana
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
74
-
-
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-azacytidine + H2O
-
209668
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209668
Arabidopsis thaliana
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209668
Escherichia coli SO5201
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azadeoxycytidine + H2O
-
209668
Arabidopsis thaliana
5-azadeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Arabidopsis thaliana
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209668
Escherichia coli SO5201
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Gallus gallus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Haemophilus influenzae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Arabidopsis thaliana
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Escherichia coli SO5201
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209668
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Arabidopsis thaliana
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Escherichia coli SO5201
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209668
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Arabidopsis thaliana
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Escherichia coli SO5201
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209668
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 32000, homodimer, SDS-PAGE
Arabidopsis thaliana
3.5.4.5
dimer
2 * 32000, homodimer, SDS-PAGE
Escherichia coli
3.5.4.5
tetramer
4 * 15000
Gallus gallus
3.5.4.5
tetramer
-
Bacillus subtilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
3
10.5
deoxycytidine as substrate, specific activity constant in this range, beyond these values irreversible inactivation occurs
Arabidopsis thaliana
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
3
10.5
deoxycytidine as substrate, beyond these extremes rapid inactivation
Arabidopsis thaliana