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Literature summary extracted from

  • Smith, A.A.; Carlow, D.C.; Wolfenden, R.; Short, S.A.
    Mutations affecting transition-state stabilization by residues coordinating zinc at the active site of cytidine deaminase (1994), Biochemistry, 33, 6468-6474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 yielding plasmid pAA5970 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 5-fluoropyrimidin-2-one ribonucleoside
-
 Escherichia coli
3.5.4.5 EDTA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.12
-
 cytidine wild-type Escherichia coli
3.5.4.5 0.2
-
 cytidine mutant H102N Escherichia coli
3.5.4.5 0.25
-
 cytidine mutant H102Q Escherichia coli
3.5.4.5 0.39
-
 cytidine mutant H102A Escherichia coli
3.5.4.5 1.5
-
 cytidine mutant C129A Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.5 Zn2+ 1 M tightly bound zinc per enzyme subunit, activated by added zinc Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 31540
-
 2 * 31540 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Escherichia coli
-
 mutant H102N
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 31540 Escherichia coli