BRENDA - Enzyme Database show

Purification and properties of Escherichia coli cytidine deaminase by amplification of the cdd gene

Kwon, T.K.; Lee, S.Y.; Kim, J.G.; Song, B.H.; Hong, S.D.; Mol. Cells 1, 281-286 (1991)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.5
cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600
Escherichia coli
3.5.4.5
cdd genes cloned and characterized
Bacillus subtilis
3.5.4.5
cdd genes cloned and characterized
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
5-bromodeoxyuridine
-
Escherichia coli
3.5.4.5
adenosine
-
Escherichia coli
3.5.4.5
Cu2+
-
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyguanosine
-
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
Fe2+
-
Escherichia coli
3.5.4.5
Fe3+
-
Escherichia coli
3.5.4.5
guanosine
-
Escherichia coli
3.5.4.5
Hg+
-
Escherichia coli
3.5.4.5
p-chloromercuribenzoate
-
Saccharomyces cerevisiae
3.5.4.5
thioglycolic acid
0.7 mM 88% relative activity, complete inhibition at 7 mM
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
uridine
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0167
-
cytidine
nonhomogenous enzyme
Escherichia coli
3.5.4.5
0.089
-
cytosine deoxyriboside
-
Escherichia coli
3.5.4.5
0.21
-
deoxycytidine
amplified gene
Escherichia coli
3.5.4.5
0.58
-
deoxycytidine
-
Escherichia coli
3.5.4.5
2.2
-
cytidine
homogenous preparation
Escherichia coli
3.5.4.5
6.6
-
cytidine
amplified gene, pH 7.0, 37°C
Escherichia coli
3.5.4.5
8.53
-
deoxycytidine
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.4.5
cytoplasm
inner-surface of cytoplasmic membrane
Escherichia coli
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
14000
-
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments
Bacillus subtilis
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
34000
-
2 * 34000, SDS-PAGE
Escherichia coli
3.5.4.5
54000
-
-
Escherichia coli
3.5.4.5
56000
-
calculated from Stokes radius
Bacillus subtilis
3.5.4.5
56000
-
calculated from Stokes radius
Escherichia coli
3.5.4.5
57000
-
gel filtration, homogenous preparation
Escherichia coli
3.5.4.5
62000
-
gel filtration, native enzyme
Escherichia coli
3.5.4.5
73000
-
gel filtration, nonhomogenous preparation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Salmonella enterica subsp. enterica serovar Typhimurium
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Geobacillus stearothermophilus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli JF611
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Bacillus subtilis
-
-
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli JF611
-
-
-
3.5.4.5
Geobacillus stearothermophilus
-
-
-
3.5.4.5
Saccharomyces cerevisiae
-
bakers' yeast
-
3.5.4.5
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
4
-
amplified gene, 5'-aza-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
4.9
-
amplified gene, 2'-thiocytidine as substrate
Escherichia coli
3.5.4.5
23.9
-
amplified gene, 5'-methylcytidine as substrate
Escherichia coli
3.5.4.5
31.8
-
amplified gene, ara-cytidine as substrate
Escherichia coli
3.5.4.5
43
-
amplified gene, cytidine as substrate
Escherichia coli
3.5.4.5
47.9
-
amplified gene, 5'-methyl-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
68.4
-
amplified gene, 2'-fluoro-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
97.6
-
amplified gene, deoxycytidine as substrate
Escherichia coli
3.5.4.5
3558
-
amplified gene
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
1-beta-D-arabinofuranosylcytosine + H2O
-
209660
Escherichia coli
?
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxycytidine + H2O
-
209660
Escherichia coli
2'-fluoro-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
2'-fluoro-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-thio-cytidine + H2O
-
209660
Escherichia coli
2'-thiouridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209660
Escherichia coli
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-aza-2'-deoxycytidine + H2O
-
209660
Escherichia coli
5-aza-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209660
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209660
Escherichia coli JF611
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Salmonella enterica subsp. enterica serovar Typhimurium
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Geobacillus stearothermophilus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Escherichia coli JF611
uridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE; 2 * 34000, SDS-PAGE
Escherichia coli
3.5.4.5
tetramer
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments
Bacillus subtilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
30
-
-
Escherichia coli
3.5.4.5
30
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
60
-
relatively labile to thermal treatment, complete inactivation by incubation for 30 min
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7
-
-
Escherichia coli
3.5.4.5
7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
6
9
reasonably stable in this range
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600
Escherichia coli
3.5.4.5
cdd genes cloned and characterized
Bacillus subtilis
3.5.4.5
cdd genes cloned and characterized
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
3,4,5,6-Tetrahydrouridine
-
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
5-bromodeoxyuridine
-
Escherichia coli
3.5.4.5
adenosine
-
Escherichia coli
3.5.4.5
Cu2+
-
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyguanosine
-
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
Fe2+
-
Escherichia coli
3.5.4.5
Fe3+
-
Escherichia coli
3.5.4.5
guanosine
-
Escherichia coli
3.5.4.5
Hg+
-
Escherichia coli
3.5.4.5
p-chloromercuribenzoate
-
Saccharomyces cerevisiae
3.5.4.5
thioglycolic acid
0.7 mM 88% relative activity, complete inhibition at 7 mM
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
uridine
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0167
-
cytidine
nonhomogenous enzyme
Escherichia coli
3.5.4.5
0.089
-
cytosine deoxyriboside
-
Escherichia coli
3.5.4.5
0.21
-
deoxycytidine
amplified gene
Escherichia coli
3.5.4.5
0.58
-
deoxycytidine
-
Escherichia coli
3.5.4.5
2.2
-
cytidine
homogenous preparation
Escherichia coli
3.5.4.5
6.6
-
cytidine
amplified gene, pH 7.0, 37°C
Escherichia coli
3.5.4.5
8.53
-
deoxycytidine
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.4.5
cytoplasm
inner-surface of cytoplasmic membrane
Escherichia coli
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
14000
-
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments
Bacillus subtilis
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
34000
-
2 * 34000, SDS-PAGE
Escherichia coli
3.5.4.5
54000
-
-
Escherichia coli
3.5.4.5
56000
-
calculated from Stokes radius
Bacillus subtilis
3.5.4.5
56000
-
calculated from Stokes radius
Escherichia coli
3.5.4.5
57000
-
gel filtration, homogenous preparation
Escherichia coli
3.5.4.5
62000
-
gel filtration, native enzyme
Escherichia coli
3.5.4.5
73000
-
gel filtration, nonhomogenous preparation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Salmonella enterica subsp. enterica serovar Typhimurium
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus subtilis
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Geobacillus stearothermophilus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli JF611
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
4
-
amplified gene, 5'-aza-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
4.9
-
amplified gene, 2'-thiocytidine as substrate
Escherichia coli
3.5.4.5
23.9
-
amplified gene, 5'-methylcytidine as substrate
Escherichia coli
3.5.4.5
31.8
-
amplified gene, ara-cytidine as substrate
Escherichia coli
3.5.4.5
43
-
amplified gene, cytidine as substrate
Escherichia coli
3.5.4.5
47.9
-
amplified gene, 5'-methyl-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
68.4
-
amplified gene, 2'-fluoro-2'-deoxycytidine as substrate
Escherichia coli
3.5.4.5
97.6
-
amplified gene, deoxycytidine as substrate
Escherichia coli
3.5.4.5
3558
-
amplified gene
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
1-beta-D-arabinofuranosylcytosine + H2O
-
209660
Escherichia coli
?
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxycytidine + H2O
-
209660
Escherichia coli
2'-fluoro-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
2'-fluoro-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-thio-cytidine + H2O
-
209660
Escherichia coli
2'-thiouridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209660
Escherichia coli
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209660
Escherichia coli JF611
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-aza-2'-deoxycytidine + H2O
-
209660
Escherichia coli
5-aza-2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209660
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209660
Escherichia coli JF611
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Salmonella enterica subsp. enterica serovar Typhimurium
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Bacillus subtilis
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Geobacillus stearothermophilus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209660
Escherichia coli JF611
uridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE; 2 * 34000, SDS-PAGE
Escherichia coli
3.5.4.5
tetramer
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
30
-
-
Escherichia coli
3.5.4.5
30
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
60
-
relatively labile to thermal treatment, complete inactivation by incubation for 30 min
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7
-
-
Escherichia coli
3.5.4.5
7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
6
9
reasonably stable in this range
Escherichia coli