BRENDA - Enzyme Database show

Further properties of cytidine deaminase and uridine phosphorylase from E. coli B

Vita, A.; Amici, A.; Lanciotti, M.; Cacciamani, T.; Magni, G.; Ital. J. Biochem. 35, 145A-147A (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
dihydrothymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymine riboside
competitive inhibition
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
-
deoxycytidine
-
Escherichia coli
3.5.4.5
1.25
-
5-methylcytidine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
56000
-
-
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli B / ATCC 11303
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methylcytidine + H2O
-
209658
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209658
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209658
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
dihydrothymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymine riboside
competitive inhibition
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
-
deoxycytidine
-
Escherichia coli
3.5.4.5
1.25
-
5-methylcytidine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
56000
-
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methylcytidine + H2O
-
209658
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209658
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209658
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209658
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE
Escherichia coli