BRENDA - Enzyme Database show

Cytidine deaminase from Escherichia coli B. Purification and enzymatic and molecular properties

Vita, A.; Amici, A.; Cacciamani, T.; Lanciotti, M.; Magni, G.; Biochemistry 24, 6020-6024 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
1-methyladenosine
competitive inhibition
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
-
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
dihydrothymidine
competitive inhibition
Escherichia coli
3.5.4.5
NH3
product inhibition
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymine riboside
competitive inhibition
Escherichia coli
3.5.4.5
uridine
product inhibition
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.18
-
cytidine
-
Escherichia coli
3.5.4.5
1.25
-
5-methylcytidine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
56000
-
gel filtration, Sephacryl S-300
Escherichia coli
3.5.4.5
66000
-
gel filtration, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli B / ATCC 11303
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity
Escherichia coli
3.5.4.5
-20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity
Escherichia coli
3.5.4.5
4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks
Escherichia coli
3.5.4.5
4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methylcytidine + H2O
-
209655
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209655
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209655
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
1-methyladenosine
competitive inhibition
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
-
Escherichia coli
3.5.4.5
deoxyadenosine
competitive inhibition
Escherichia coli
3.5.4.5
deoxyuridine
competitive inhibition
Escherichia coli
3.5.4.5
dihydrothymidine
competitive inhibition
Escherichia coli
3.5.4.5
NH3
product inhibition
Escherichia coli
3.5.4.5
thymidine
competitive inhibition
Escherichia coli
3.5.4.5
thymine riboside
competitive inhibition
Escherichia coli
3.5.4.5
uridine
product inhibition
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.18
-
cytidine
-
Escherichia coli
3.5.4.5
1.25
-
5-methylcytidine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
33000
-
2 * 33000, SDS-PAGE
Escherichia coli
3.5.4.5
56000
-
gel filtration, Sephacryl S-300
Escherichia coli
3.5.4.5
66000
-
gel filtration, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity
Escherichia coli
3.5.4.5
-20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity
Escherichia coli
3.5.4.5
4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks
Escherichia coli
3.5.4.5
4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5-methylcytidine + H2O
-
209655
Escherichia coli
5-methyluridine + NH3
-
-
-
?
3.5.4.5
5-methylcytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
5-methyluridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209655
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209655
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209655
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 33000, SDS-PAGE
Escherichia coli