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Literature summary extracted from

  • Ashley, G.W.; Bartlett, P.A.
    Purification and properties of cytidine deaminase from escherichia coli (1984), J. Biol. Chem., 259, 13615-13620.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 5-(Chloromercuri)cytidine
-
 Escherichia coli
3.5.4.5 5-(Chloromercuri)cytidine
-
 Homo sapiens
3.5.4.5 Borate
-
 Escherichia coli
3.5.4.5 mercaptoethanol
-
 Escherichia coli
3.5.4.5 Mersalyl acid
-
 Escherichia coli
3.5.4.5 Mersalyl acid
-
 Mus musculus
3.5.4.5 Mersalyl acid
-
 Saccharomyces cerevisiae
3.5.4.5 p-mercuribenzoate enzyme from leukemic spleen, enzyme from normal spleen is much less sensitive Mus musculus
3.5.4.5 p-mercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.058
-
 2'-deoxycytidine
-
 Escherichia coli
3.5.4.5 0.07
-
 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside
-
 Escherichia coli
3.5.4.5 0.22
-
 cytidine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 35000
-
 2 * 35000, SDS-PAGE Escherichia coli
3.5.4.5 51000
-
 human granulocytes Homo sapiens
3.5.4.5 54000
-
 gel filtration Escherichia coli
3.5.4.5 57000
-
-
 Saccharomyces cerevisiae
3.5.4.5 74000
-
 spleen Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Saccharomyces cerevisiae
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Ovis aries
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Zea mays
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli B / ATCC 11303
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Escherichia coli
-
-
-
3.5.4.5 Escherichia coli B / ATCC 11303
-
-
-
3.5.4.5 Homo sapiens
-
 human
-
3.5.4.5 Mus musculus
-
 mouse
-
3.5.4.5 Mus musculus
-
 BALB/c
-
3.5.4.5 Mus musculus BALB/c
-
 BALB/c
-
3.5.4.5 Ovis aries
-
-
-
3.5.4.5 Saccharomyces cerevisiae
-
-
-
3.5.4.5 Zea mays
-
 corn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Mus musculus
3.5.4.5
-
 Escherichia coli
3.5.4.5
-
 Homo sapiens
3.5.4.5
-
 Saccharomyces cerevisiae
3.5.4.5
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.5 kidney
-
 Mus musculus
-
3.5.4.5 kidney
-
 Homo sapiens
-
3.5.4.5 liver
-
 Mus musculus
-
3.5.4.5 liver
-
 Homo sapiens
-
3.5.4.5 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 118
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.4.5 4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle Escherichia coli
3.5.4.5 4°C, stable for several days, may be kept for months without significant loss of activity if frozen Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 2'-deoxycytidine + H2O
-
 Mus musculus 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Escherichia coli 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Homo sapiens 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Saccharomyces cerevisiae 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Escherichia coli B / ATCC 11303 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Mus musculus BALB/c 2'-deoxyuridine + NH3
-
 ?
3.5.4.5 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
 Escherichia coli ?
-
 ?
3.5.4.5 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
 Homo sapiens ?
-
 ?
3.5.4.5 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
3.5.4.5 2'-O-methylcytidine + H2O
-
 Escherichia coli ?
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Escherichia coli 5-azauridine + NH3
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Homo sapiens 5-azauridine + NH3
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Escherichia coli B / ATCC 11303 5-azauridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Saccharomyces cerevisiae uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Ovis aries uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Zea mays uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli B / ATCC 11303 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Mus musculus uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Escherichia coli uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Homo sapiens uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Escherichia coli B / ATCC 11303 uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Mus musculus BALB/c uridine arabinoside + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 35000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.4.5 CDase
-
 Escherichia coli