BRENDA - Enzyme Database show

Purification and properties of cytidine deaminase from escherichia coli

Ashley, G.W.; Bartlett, P.A.; J. Biol. Chem. 259, 13615-13620 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5-(Chloromercuri)cytidine
-
Escherichia coli
3.5.4.5
5-(Chloromercuri)cytidine
-
Homo sapiens
3.5.4.5
Borate
-
Escherichia coli
3.5.4.5
mercaptoethanol
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Mus musculus
3.5.4.5
Mersalyl acid
-
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
enzyme from leukemic spleen, enzyme from normal spleen is much less sensitive
Mus musculus
3.5.4.5
p-mercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.058
-
2'-deoxycytidine
-
Escherichia coli
3.5.4.5
0.07
-
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside
-
Escherichia coli
3.5.4.5
0.22
-
cytidine
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
35000
-
2 * 35000, SDS-PAGE
Escherichia coli
3.5.4.5
51000
-
human granulocytes
Homo sapiens
3.5.4.5
54000
-
gel filtration
Escherichia coli
3.5.4.5
57000
-
-
Saccharomyces cerevisiae
3.5.4.5
74000
-
spleen
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Zea mays
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli B / ATCC 11303
-
-
-
3.5.4.5
Homo sapiens
-
human
-
3.5.4.5
Mus musculus
-
BALB/c; mouse
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
3.5.4.5
Ovis aries
-
-
-
3.5.4.5
Saccharomyces cerevisiae
-
-
-
3.5.4.5
Zea mays
-
corn
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Mus musculus
3.5.4.5
-
Ovis aries
3.5.4.5
-
Saccharomyces cerevisiae
3.5.4.5
-
Escherichia coli
3.5.4.5
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
liver
-
Ovis aries
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
118
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle
Escherichia coli
3.5.4.5
4°C, stable for several days, may be kept for months without significant loss of activity if frozen
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Escherichia coli
?
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Homo sapiens
?
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Escherichia coli B / ATCC 11303
?
-
-
-
?
3.5.4.5
2'-O-methylcytidine + H2O
-
209653
Escherichia coli
?
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
5-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Zea mays
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Escherichia coli B / ATCC 11303
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 35000, SDS-PAGE
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5-(Chloromercuri)cytidine
-
Escherichia coli
3.5.4.5
5-(Chloromercuri)cytidine
-
Homo sapiens
3.5.4.5
Borate
-
Escherichia coli
3.5.4.5
mercaptoethanol
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Mus musculus
3.5.4.5
Mersalyl acid
-
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
enzyme from leukemic spleen, enzyme from normal spleen is much less sensitive
Mus musculus
3.5.4.5
p-mercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.058
-
2'-deoxycytidine
-
Escherichia coli
3.5.4.5
0.07
-
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside
-
Escherichia coli
3.5.4.5
0.22
-
cytidine
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
35000
-
2 * 35000, SDS-PAGE
Escherichia coli
3.5.4.5
51000
-
human granulocytes
Homo sapiens
3.5.4.5
54000
-
gel filtration
Escherichia coli
3.5.4.5
57000
-
-
Saccharomyces cerevisiae
3.5.4.5
74000
-
spleen
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Zea mays
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Mus musculus
3.5.4.5
-
Ovis aries
3.5.4.5
-
Saccharomyces cerevisiae
3.5.4.5
-
Escherichia coli
3.5.4.5
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
liver
-
Ovis aries
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
118
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle
Escherichia coli
3.5.4.5
4°C, stable for several days, may be kept for months without significant loss of activity if frozen
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209653
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Escherichia coli
?
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Homo sapiens
?
-
-
-
?
3.5.4.5
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O
-
209653
Escherichia coli B / ATCC 11303
?
-
-
-
?
3.5.4.5
2'-O-methylcytidine + H2O
-
209653
Escherichia coli
?
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
5-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Zea mays
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209653
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Escherichia coli B / ATCC 11303
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209653
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
dimer
2 * 35000, SDS-PAGE
Escherichia coli