BRENDA - Enzyme Database show

Characteristics of cytidine aminohydrolase activity in Trypanosoma cruzi and Crithidia fasciculata

Kidder, G.W.; J. Protozool. 31, 298-300 (1984)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
5-Bromouridine
-
Crithidia fasciculata
3.5.4.5
cytidine
-
Crithidia fasciculata
3.5.4.5
cytidine
-
Trypanosoma cruzi
3.5.4.5
Orotidine
-
Crithidia fasciculata
3.5.4.5
thymidine
-
Crithidia fasciculata
3.5.4.5
uridine
-
Crithidia fasciculata
General Stability
EC Number
General Stability
Organism
3.5.4.5
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions
Crithidia fasciculata
3.5.4.5
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions
Trypanosoma cruzi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
p-mercuribenzoate
-
Crithidia fasciculata
3.5.4.5
p-mercuribenzoate
-
Trypanosoma cruzi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
0.1
deoxycytidine
-
Crithidia fasciculata
3.5.4.5
1.8
1.9
cytidine
radioactive labeled substrate
Trypanosoma cruzi
3.5.4.5
1.8
1.9
deoxycytidine
radioactive labeled substrate
Trypanosoma cruzi
3.5.4.5
2.8
3
cytidine
-
Crithidia fasciculata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
45000
-
gel filtration
Crithidia fasciculata
3.5.4.5
90000
-
gel filtration
Trypanosoma cruzi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Crithidia fasciculata
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Trypanosoma cruzi
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Crithidia fasciculata
-
mosquito parasite
-
3.5.4.5
Trypanosoma cruzi
-
human pathogen, Culbertson strain, epimastigotes, ATCC 30013
-
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
Crithidia fasciculata
3.5.4.5
-20°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
Trypanosoma cruzi
3.5.4.5
4°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
Crithidia fasciculata
3.5.4.5
4°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
Trypanosoma cruzi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
cytidine + H2O
-
209652
Crithidia fasciculata
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209652
Trypanosoma cruzi
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209652
Crithidia fasciculata
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209652
Trypanosoma cruzi
deoxyuridine + NH3
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
6.5
8.5
-
Crithidia fasciculata
3.5.4.5
6.5
8.5
-
Trypanosoma cruzi
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
5-Bromouridine
-
Crithidia fasciculata
3.5.4.5
cytidine
-
Crithidia fasciculata
3.5.4.5
cytidine
-
Trypanosoma cruzi
3.5.4.5
Orotidine
-
Crithidia fasciculata
3.5.4.5
thymidine
-
Crithidia fasciculata
3.5.4.5
uridine
-
Crithidia fasciculata
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.4.5
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions
Crithidia fasciculata
3.5.4.5
unstable in air, even in the frozen state, stabilization achieved under anaerobic conditions
Trypanosoma cruzi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
p-mercuribenzoate
-
Crithidia fasciculata
3.5.4.5
p-mercuribenzoate
-
Trypanosoma cruzi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.09
0.1
deoxycytidine
-
Crithidia fasciculata
3.5.4.5
1.8
1.9
cytidine
radioactive labeled substrate
Trypanosoma cruzi
3.5.4.5
1.8
1.9
deoxycytidine
radioactive labeled substrate
Trypanosoma cruzi
3.5.4.5
2.8
3
cytidine
-
Crithidia fasciculata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
45000
-
gel filtration
Crithidia fasciculata
3.5.4.5
90000
-
gel filtration
Trypanosoma cruzi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Crithidia fasciculata
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Trypanosoma cruzi
-
uridine + NH3
-
-
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
-20°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
Crithidia fasciculata
3.5.4.5
-20°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
Trypanosoma cruzi
3.5.4.5
4°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h
Crithidia fasciculata
3.5.4.5
4°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h
Trypanosoma cruzi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
cytidine + H2O
-
209652
Crithidia fasciculata
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209652
Trypanosoma cruzi
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209652
Crithidia fasciculata
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209652
Trypanosoma cruzi
deoxyuridine + NH3
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
6.5
8.5
-
Crithidia fasciculata
3.5.4.5
6.5
8.5
-
Trypanosoma cruzi