BRENDA - Enzyme Database show

Cytidine deaminase from leukemic mouse spleen

Rothman, I.K.; Malathi, V.G.; Silber, R.; Methods Enzymol. 51, 408-412 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
dithiothreitol
-
Mus musculus
3.5.4.5
EDTA
-
Mus musculus
3.5.4.5
GSH
-
Mus musculus
Application
EC Number
Application
Commentary
Organism
3.5.4.5
medicine
enzyme limits the effectiveness of the antineoplastic agent cytosine arabinoside or aracytidine in the treatment of acute myeloblastic leukemias, chemotherapeutic use of the inhibitor THU to protect antileukemic agents from deaminase attack, inhibitors of cytidine deaminase prolongs the plasma halflife of antitumor or antiviral drugs
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
Cu2+
-
Homo sapiens
3.5.4.5
Cu2+
-
Mus musculus
3.5.4.5
Hg+
-
Homo sapiens
3.5.4.5
Hg+
-
Mus musculus
3.5.4.5
N-ethylmaleimide
-
Homo sapiens
3.5.4.5
N-ethylmaleimide
-
Mus musculus
3.5.4.5
p-mercuribenzoate
highly sensitive
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.063
-
cytidine
normal and leukemic spleen enzyme
Mus musculus
3.5.4.5
0.079
-
deoxycytidine
leukemic spleen enzyme
Mus musculus
3.5.4.5
0.34
-
deoxycytidine
leukemic spleen enzyme
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.5
Mn2+
slight activation
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
74000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Homo sapiens
-
HeLa cells; human
-
3.5.4.5
Mus musculus
-
BALB/c; mouse; Swiss-Webster strain DBA/2
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
granulocyte
-
Homo sapiens
-
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
leukocyte
-
Homo sapiens
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
3.5.4.5
spleen
normal and leukemic spleen
Mus musculus
-
3.5.4.5
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
90
-
-
Mus musculus
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
-20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity
Mus musculus
3.5.4.5
4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209649
Mus musculus
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209649
Homo sapiens
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-bromodeoxycytidine + H2O
-
209649
Mus musculus
5-bromodeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209649
Mus musculus
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209649
Homo sapiens
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
209649
Mus musculus
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
209649
Homo sapiens
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates
209649
Mus musculus
?
-
-
-
-
3.5.4.5
additional information
cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates
209649
Mus musculus BALB/c
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
50
-
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
5
8
-
Mus musculus
3.5.4.5
7.2
-
leukemic spleen enzyme
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
dithiothreitol
-
Mus musculus
3.5.4.5
EDTA
-
Mus musculus
3.5.4.5
GSH
-
Mus musculus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.4.5
medicine
enzyme limits the effectiveness of the antineoplastic agent cytosine arabinoside or aracytidine in the treatment of acute myeloblastic leukemias, chemotherapeutic use of the inhibitor THU to protect antileukemic agents from deaminase attack, inhibitors of cytidine deaminase prolongs the plasma halflife of antitumor or antiviral drugs
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
Cu2+
-
Homo sapiens
3.5.4.5
Cu2+
-
Mus musculus
3.5.4.5
Hg+
-
Homo sapiens
3.5.4.5
Hg+
-
Mus musculus
3.5.4.5
N-ethylmaleimide
-
Homo sapiens
3.5.4.5
N-ethylmaleimide
-
Mus musculus
3.5.4.5
p-mercuribenzoate
highly sensitive
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.063
-
cytidine
normal and leukemic spleen enzyme
Mus musculus
3.5.4.5
0.079
-
deoxycytidine
leukemic spleen enzyme
Mus musculus
3.5.4.5
0.34
-
deoxycytidine
leukemic spleen enzyme
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.5
Mn2+
slight activation
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
74000
-
gel filtration
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
granulocyte
-
Homo sapiens
-
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
leukocyte
-
Homo sapiens
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
3.5.4.5
spleen
normal and leukemic spleen
Mus musculus
-
3.5.4.5
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
90
-
-
Mus musculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
-20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity
Mus musculus
3.5.4.5
4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209649
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209649
Mus musculus
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209649
Homo sapiens
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5-bromodeoxycytidine + H2O
-
209649
Mus musculus
5-bromodeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209649
Mus musculus
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209649
Homo sapiens
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
209649
Mus musculus
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
5-iododeoxycytidine + H2O
-
209649
Homo sapiens
5-iododeoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209649
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Homo sapiens
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209649
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209649
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates
209649
Mus musculus
?
-
-
-
-
3.5.4.5
additional information
cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates
209649
Mus musculus BALB/c
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.5
50
-
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
5
8
-
Mus musculus
3.5.4.5
7.2
-
leukemic spleen enzyme
Mus musculus