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Literature summary extracted from
- Cytidine deaminase from leukemic mouse spleen (1978), Methods Enzymol., 51, 408-412.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.5 | dithiothreitol | - |
Mus musculus |  |
| 3.5.4.5 | EDTA | - |
Mus musculus | |
| 3.5.4.5 | GSH | - |
Mus musculus | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.4.5 | medicine | enzyme limits the effectiveness of the antineoplastic agent cytosine arabinoside or aracytidine in the treatment of acute myeloblastic leukemias, chemotherapeutic use of the inhibitor THU to protect antileukemic agents from deaminase attack, inhibitors of cytidine deaminase prolongs the plasma halflife of antitumor or antiviral drugs | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.5 | Cu2+ | - |
Homo sapiens | |
| 3.5.4.5 | Cu2+ | - |
Mus musculus | |
| 3.5.4.5 | Hg+ | - |
Homo sapiens | |
| 3.5.4.5 | Hg+ | - |
Mus musculus | |
| 3.5.4.5 | N-ethylmaleimide | - |
Homo sapiens | |
| 3.5.4.5 | N-ethylmaleimide | - |
Mus musculus | |
| 3.5.4.5 | p-mercuribenzoate | highly sensitive | Mus musculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.5 | 0.063 | - |
cytidine | normal and leukemic spleen enzyme | Mus musculus | |
| 3.5.4.5 | 0.079 | - |
deoxycytidine | leukemic spleen enzyme | Mus musculus | |
| 3.5.4.5 | 0.34 | - |
deoxycytidine | leukemic spleen enzyme | Mus musculus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.5 | Mn2+ | slight activation | Mus musculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 74000 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.5 | cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Homo sapiens | - |
uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.5 | Homo sapiens | - |
human | - |
| 3.5.4.5 | Homo sapiens | - |
HeLa cells | - |
| 3.5.4.5 | Mus musculus | - |
mouse | - |
| 3.5.4.5 | Mus musculus | - |
BALB/c | - |
| 3.5.4.5 | Mus musculus | - |
Swiss-Webster strain DBA/2 | - |
| 3.5.4.5 | Mus musculus BALB/c | - |
BALB/c | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.5 | - |
Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.5 | granulocyte | - |
Homo sapiens | - |
| 3.5.4.5 | kidney | - |
Mus musculus | - |
| 3.5.4.5 | kidney | - |
Homo sapiens | - |
| 3.5.4.5 | leukocyte | - |
Homo sapiens | - |
| 3.5.4.5 | liver | - |
Mus musculus | - |
| 3.5.4.5 | liver | - |
Homo sapiens | - |
| 3.5.4.5 | spleen | - |
Homo sapiens | - |
| 3.5.4.5 | spleen | normal and leukemic spleen | Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 90 | - |
- |
Mus musculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.5 | -20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity | Mus musculus |
| 3.5.4.5 | 4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mus musculus | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Homo sapiens | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 2'-deoxycytidine + H2O | - |
Mus musculus BALB/c | 2'-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 5'-methyl-2'-deoxycytidine + H2O | - |
Mus musculus | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 5'-methyl-2'-deoxycytidine + H2O | - |
Homo sapiens | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 5-bromodeoxycytidine + H2O | - |
Mus musculus | 5-bromodeoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 5-iodocytidine + H2O | - |
Mus musculus | 5-iodouridine + NH3 | - |
? | |
| 3.5.4.5 | 5-iodocytidine + H2O | - |
Homo sapiens | 5-iodouridine + NH3 | - |
? | |
| 3.5.4.5 | 5-iododeoxycytidine + H2O | - |
Mus musculus | 5-iododeoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | 5-iododeoxycytidine + H2O | - |
Homo sapiens | 5-iododeoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
| 3.5.4.5 | cytosine arabinoside + H2O | - |
Mus musculus | uridine arabinoside + NH3 | - |
? | |
| 3.5.4.5 | cytosine arabinoside + H2O | - |
Homo sapiens | uridine arabinoside + NH3 | - |
? | |
| 3.5.4.5 | cytosine arabinoside + H2O | - |
Mus musculus BALB/c | uridine arabinoside + NH3 | - |
? | |
| 3.5.4.5 | deoxycytidine + H2O | - |
Mus musculus | deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | deoxycytidine + H2O | - |
Homo sapiens | deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | deoxycytidine + H2O | - |
Mus musculus BALB/c | deoxyuridine + NH3 | - |
? | |
| 3.5.4.5 | additional information | cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates | Mus musculus | ? | - |
? | |
| 3.5.4.5 | additional information | cytosine, 5-methylcytosine, 6-methylaminopurine, CMP, dCMP, CDP, dCDP, CTP and dCTP are no substrates | Mus musculus BALB/c | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 50 | - |
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.5 | 5 | 8 | - |
Mus musculus |
| 3.5.4.5 | 7.2 | - |
leukemic spleen enzyme | Mus musculus |
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