BRENDA - Enzyme Database show

The purification and properties of cytidine deaminase from Escherichia coli

Hosono, H.; Kuno, S.; J. Biochem. 74, 797-803 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
EDTA
10 mM, activity 108%
Escherichia coli
3.5.4.5
EDTA
-
Mus musculus
3.5.4.5
iodoacetic acid
0.10 mM, activity 105%
Escherichia coli
3.5.4.5
N-ethylmaleimide
0.10-0.20 mM, activity 114%
Escherichia coli
3.5.4.5
p-chloromercuribenzoate
0.105 mM, activity 112%
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5-bromodeoxyuridine
1.0 mM, 31% inhibition
Escherichia coli
3.5.4.5
adenosine
4.0 mM, 22% inhibition
Escherichia coli
3.5.4.5
Cu2+
-
Escherichia coli
3.5.4.5
Cu2+
-
Mus musculus
3.5.4.5
deoxyadenosine
3.0 mM, 32% inhibition
Escherichia coli
3.5.4.5
deoxyguanosine
1.0 mM, 13% inhibition
Escherichia coli
3.5.4.5
deoxyuridine
1.0 mM, 32% inhibition
Escherichia coli
3.5.4.5
dTTP
-
Ovis aries
3.5.4.5
dTTP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
-
Mus musculus
3.5.4.5
dUTP
-
Mus musculus
3.5.4.5
dUTP
-
Ovis aries
3.5.4.5
Fe2+
-
Escherichia coli
3.5.4.5
Fe3+
-
Escherichia coli
3.5.4.5
guanosine
1.0 mM, 4% inhibition
Escherichia coli
3.5.4.5
Hg+
-
Escherichia coli
3.5.4.5
Hg+
-
Mus musculus
3.5.4.5
mercaptoethanol
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Mus musculus
3.5.4.5
Mersalyl acid
-
Saccharomyces cerevisiae
3.5.4.5
thymidine
1.0 mM, 21% inhibition
Escherichia coli
3.5.4.5
uracil arabinoside
4.0 mM, 48% inhibition
Escherichia coli
3.5.4.5
Urea
above 2 M, reversible inactivation
Escherichia coli
3.5.4.5
uridine
4.0 mM, 20% inhibition
Escherichia coli
3.5.4.5
Zn2+
above 10 mM
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.06
-
5-Methyldeoxycytidine
-
Escherichia coli
3.5.4.5
0.0853
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.087
-
5'-fluoro-5'-deoxycytidine
-
Escherichia coli
3.5.4.5
0.167
-
cytidine
-
Escherichia coli
3.5.4.5
0.26
-
cytosine arabinoside
-
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.5
Mg2+
1 mM, activity 101%
Escherichia coli
3.5.4.5
Mn2+
10 mM, activity 106%
Escherichia coli
3.5.4.5
Mn2+
-
Mus musculus
3.5.4.5
Zn2+
1 mM, activity 103%
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
54000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus cereus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli Y-70-272
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Bacillus cereus
-
-
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli Y-70-272
-
-
-
3.5.4.5
Mus musculus
-
BALB/c; mouse
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
3.5.4.5
Ovis aries
-
-
-
3.5.4.5
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
liver
-
Ovis aries
-
3.5.4.5
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
9.53
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5'-fluoro-5'-deoxycytidine + H2O
-
209644
Escherichia coli
5'-fluoro-5'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-fluoro-5'-deoxycytidine + H2O
-
209644
Escherichia coli Y-70-272
5'-fluoro-5'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Mus musculus
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Escherichia coli
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Mus musculus BALB/c
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Bacillus cereus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Escherichia coli Y-70-272
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Escherichia coli Y-70-272
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Escherichia coli Y-70-272
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
cytosine and cytidine nucleosides are not deaminated by this enzyme
209644
Escherichia coli
?
-
-
-
-
3.5.4.5
additional information
cytosine and cytidine nucleosides are not deaminated by this enzyme
209644
Escherichia coli Y-70-272
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
monomer
1 * 54000
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
6
7.5
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.5
EDTA
10 mM, activity 108%
Escherichia coli
3.5.4.5
EDTA
-
Mus musculus
3.5.4.5
iodoacetic acid
0.10 mM, activity 105%
Escherichia coli
3.5.4.5
N-ethylmaleimide
0.10-0.20 mM, activity 114%
Escherichia coli
3.5.4.5
p-chloromercuribenzoate
0.105 mM, activity 112%
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5-bromodeoxyuridine
1.0 mM, 31% inhibition
Escherichia coli
3.5.4.5
adenosine
4.0 mM, 22% inhibition
Escherichia coli
3.5.4.5
Cu2+
-
Escherichia coli
3.5.4.5
Cu2+
-
Mus musculus
3.5.4.5
deoxyadenosine
3.0 mM, 32% inhibition
Escherichia coli
3.5.4.5
deoxyguanosine
1.0 mM, 13% inhibition
Escherichia coli
3.5.4.5
deoxyuridine
1.0 mM, 32% inhibition
Escherichia coli
3.5.4.5
dTTP
-
Ovis aries
3.5.4.5
dTTP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
-
Mus musculus
3.5.4.5
dUTP
-
Mus musculus
3.5.4.5
dUTP
-
Ovis aries
3.5.4.5
Fe2+
-
Escherichia coli
3.5.4.5
Fe3+
-
Escherichia coli
3.5.4.5
guanosine
1.0 mM, 4% inhibition
Escherichia coli
3.5.4.5
Hg+
-
Escherichia coli
3.5.4.5
Hg+
-
Mus musculus
3.5.4.5
mercaptoethanol
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Escherichia coli
3.5.4.5
Mersalyl acid
-
Mus musculus
3.5.4.5
Mersalyl acid
-
Saccharomyces cerevisiae
3.5.4.5
thymidine
1.0 mM, 21% inhibition
Escherichia coli
3.5.4.5
uracil arabinoside
4.0 mM, 48% inhibition
Escherichia coli
3.5.4.5
Urea
above 2 M, reversible inactivation
Escherichia coli
3.5.4.5
uridine
4.0 mM, 20% inhibition
Escherichia coli
3.5.4.5
Zn2+
above 10 mM
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.06
-
5-Methyldeoxycytidine
-
Escherichia coli
3.5.4.5
0.0853
-
deoxycytidine
-
Escherichia coli
3.5.4.5
0.087
-
5'-fluoro-5'-deoxycytidine
-
Escherichia coli
3.5.4.5
0.167
-
cytidine
-
Escherichia coli
3.5.4.5
0.26
-
cytosine arabinoside
-
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.5
Mg2+
1 mM, activity 101%
Escherichia coli
3.5.4.5
Mn2+
10 mM, activity 106%
Escherichia coli
3.5.4.5
Mn2+
-
Mus musculus
3.5.4.5
Zn2+
1 mM, activity 103%
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
54000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Ovis aries
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Bacillus cereus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli Y-70-272
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
liver
-
Ovis aries
-
3.5.4.5
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
9.53
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
5'-fluoro-5'-deoxycytidine + H2O
-
209644
Escherichia coli
5'-fluoro-5'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-fluoro-5'-deoxycytidine + H2O
-
209644
Escherichia coli Y-70-272
5'-fluoro-5'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Mus musculus
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Escherichia coli
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-methyl-2'-deoxycytidine + H2O
-
209644
Mus musculus BALB/c
5'-methyl-2-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Ovis aries
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Bacillus cereus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209644
Escherichia coli Y-70-272
uridine + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Mus musculus
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Escherichia coli
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Mus musculus BALB/c
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
cytosine arabinoside + H2O
-
209644
Escherichia coli Y-70-272
uridine arabinoside + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209644
Escherichia coli Y-70-272
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
cytosine and cytidine nucleosides are not deaminated by this enzyme
209644
Escherichia coli
?
-
-
-
-
3.5.4.5
additional information
cytosine and cytidine nucleosides are not deaminated by this enzyme
209644
Escherichia coli Y-70-272
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
monomer
1 * 54000
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
6
7.5
-
Escherichia coli