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Literature summary extracted from

  • Cohen, R.M.; Wolfenden, R.
    Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine (1971), J. Biol. Chem., 246, 7561-7565.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 3,4,5,6-Tetrahydrouridine potent competitive inhibitor Escherichia coli
3.5.4.5 3,4,5,6-Tetrahydrouridine potent competitive inhibitor Homo sapiens
3.5.4.5 3,4,5,6-Tetrahydrouridine potent competitive inhibitor Mus musculus
3.5.4.5 5,6-Dihydrouridine competitive inhibition Escherichia coli
3.5.4.5 5,6-Dihydrouridine
-
 Homo sapiens
3.5.4.5 uridine product inhibition Escherichia coli
3.5.4.5 uridine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.2
-
 cytidine at pH 7.5 with H2O and pH 8.4 with H2O or D2O Escherichia coli
3.5.4.5 0.208
-
 cytidine at pH 7.5 with D2O Escherichia coli
3.5.4.5 0.233
-
 cytidine at pH 7.1 with D2O Escherichia coli
3.5.4.5 0.238
-
 cytidine at pH 7.1 with D2O Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 73000
-
 gel filtration, nonhomogenous preparation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Saccharomyces cerevisiae
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Ovis aries
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli B / ATCC 11303
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Escherichia coli
-
-
-
3.5.4.5 Escherichia coli B / ATCC 11303
-
-
-
3.5.4.5 Homo sapiens
-
 human
-
3.5.4.5 Mus musculus
-
 mouse
-
3.5.4.5 Mus musculus
-
 BALB/c
-
3.5.4.5 Mus musculus BALB/c
-
 BALB/c
-
3.5.4.5 Ovis aries
-
-
-
3.5.4.5 Saccharomyces cerevisiae
-
 bakers' yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.5 kidney
-
 Mus musculus
-
3.5.4.5 kidney
-
 Homo sapiens
-
3.5.4.5 liver
-
 Mus musculus
-
3.5.4.5 liver
-
 Homo sapiens
-
3.5.4.5 liver
-
 Ovis aries
-

Storage Stability

EC Number Storage Stability Organism
3.5.4.5 4°C, reasonably stable, retains 55% of its activity after 3 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Saccharomyces cerevisiae uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Ovis aries uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli B / ATCC 11303 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?
3.5.4.5 N4-methylcytidine + H2O
-
 Escherichia coli uridine + methylamine
-
 r
3.5.4.5 N4-methylcytidine + H2O
-
 Escherichia coli B / ATCC 11303 uridine + methylamine
-
 r

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.5 6 11
-
 Escherichia coli