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Literature summary extracted from

  • Yuan, G.; Bin, J.C.; McKay, D.J.; Snyder, F.F.
    Cloning and characterization of human guanine deaminase. Purification and partial amino acid sequence of the mouse protein (1999), J. Biol. Chem., 274, 8175-8180.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.3 medicine enzyme has been implicated in cancer, liver disease, saturine gout, psoriasis and autoimmune disease, histochemical method for demonstration of guanase should be useful clinically, guanase activity increases significantly in lung and gastric cancer tissues or in patients with liver diseases like hepatitis, guanase is a biochemical indicator of rejection in liver transplant recipients, serum enzyme activity is the most sensitive indicator of liver disease, inhibition of guanase has beneficial implications in cancer chemotherapy, an inhibitor as chemotherapeutic agent selectively inhibit the growth of rapidly proliferating cancerous cells via depletion of the purine nucleotide pool Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.3 cloned from kidney and brain cDNA libraries, PCR, subcloned into bacterial expression vector pMAL-c2, expressed in mice DH5-alpha cells and purified Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.3 p-hydroxymercuribenzoate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.0095
-
 guanine recombinant enzyme, expressed in DH5-alpha cells Homo sapiens
3.5.4.3 0.011
-
 guanine
-
 Sus sp.
3.5.4.3 0.0153
-
 guanine
-
 Homo sapiens
3.5.4.3 0.0227
-
 guanine
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.3 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.3 Mn2+ addition results in 2fold increase of activity Homo sapiens
3.5.4.3 Zn2+ association of 1 atom per enzyme monomer Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Mus musculus
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Homo sapiens
3.5.4.3 50000
-
 4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE Mus musculus
3.5.4.3 51040
-
 calculated from cDNA corresponding to an open reading frame Homo sapiens
3.5.4.3 55000
-
 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Sus sp.
3.5.4.3 55000
-
 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Homo sapiens
3.5.4.3 59000
-
 2 * 59000, SDS-PAGE Homo sapiens
3.5.4.3 100000
-
 gel filtration Homo sapiens
3.5.4.3 200000
-
-
 Homo sapiens
3.5.4.3 200000
-
 erythrocytic enzyme, 5% of the protein present Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.3 guanine + H2O Mus musculus
-
 xanthine + NH3
-
 ir
3.5.4.3 guanine + H2O Sus sp.
-
 xanthine + NH3
-
 ir
3.5.4.3 guanine + H2O Homo sapiens
-
 xanthine + NH3
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Homo sapiens Q9Y2T3 human
-
3.5.4.3 Mus musculus
-
 mouse
-
3.5.4.3 Sus sp.
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3
-
 Mus musculus
3.5.4.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.3 brain
-
 Mus musculus
-
3.5.4.3 brain
-
 Sus sp.
-
3.5.4.3 brain
-
 Homo sapiens
-
3.5.4.3 erythrocyte
-
 Mus musculus
-
3.5.4.3 intestine small intestine Mus musculus
-
3.5.4.3 kidney
-
 Homo sapiens
-
3.5.4.3 liver
-
 Mus musculus
-
3.5.4.3 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.3 9.53
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 guanine + H2O
-
 Mus musculus xanthine + NH3
-
 ir
3.5.4.3 guanine + H2O
-
 Sus sp. xanthine + NH3
-
 ir
3.5.4.3 guanine + H2O
-
 Homo sapiens xanthine + NH3
-
 ir

Subunits

EC Number Subunits Comment Organism
3.5.4.3 dimer 2 * 50000, SDS-PAGE Mus musculus
3.5.4.3 dimer 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 dimer 2 * 50000, SDS-PAGE Homo sapiens
3.5.4.3 dimer 2 * 59000, SDS-PAGE Homo sapiens
3.5.4.3 dimer 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Sus sp.
3.5.4.3 dimer 2 * 55000, can be converted into monomeric form by addition of sulfhydryl groups Homo sapiens
3.5.4.3 tetramer 4 * 50000, erythrocytic enzyme, 5% of the protein present, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.5.4.3 human guanine deaminase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.3 7
-
 recombinant protein, expressed in mice DH5-alpha cells, Km remains constant between pH 6.5 and pH 7.5 Homo sapiens