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Literature summary extracted from
- Rabbit liver guanine deaminase. Chemical, physical, and kinetic properties (1974), J. Biol. Chem., 249, 3862-3866.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.5.4.3 | stabilized by 2-mercaptoethanol, labile to heat | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.3 | 5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus |  |
| 3.5.4.3 | 5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
| 3.5.4.3 | 5-aminoimidazole-4-carboxamide ribonucleoside | - |
Oryctolagus cuniculus | |
| 3.5.4.3 | 5-aminoimidazole-4-carboxamide ribonucleoside | - |
Rattus norvegicus | |
| 3.5.4.3 | iodoacetamide | - |
Oryctolagus cuniculus | |
| 3.5.4.3 | iodoacetic acid | - |
Oryctolagus cuniculus | |
| 3.5.4.3 | p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
| 3.5.4.3 | p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
| 3.5.4.3 | tetrahydrofolic acid | - |
Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.3 | 0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
| 3.5.4.3 | 0.0182 | - |
guanine | buffer with 50% D2O | Oryctolagus cuniculus | |
| 3.5.4.3 | 0.025 | - |
guanine | buffer with 100% D2O | Oryctolagus cuniculus | |
| 3.5.4.3 | 0.333 | - |
8-Azaguanine | at pH 6 | Oryctolagus cuniculus | |
| 3.5.4.3 | 0.8 | - |
6-thioguanine | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.3 | 54000 | - |
SDS-PAGE | Oryctolagus cuniculus |
| 3.5.4.3 | 55000 | - |
liver, single polypeptide chain, gel filtration | Rattus norvegicus |
| 3.5.4.3 | 55000 | - |
liver, single polypeptide chain, gel filtration | Oryctolagus cuniculus |
| 3.5.4.3 | 56000 | - |
liver enzyme, gel filtration | Oryctolagus cuniculus |
| 3.5.4.3 | 170000 | - |
- |
Oryctolagus cuniculus |
| 3.5.4.3 | 525000 | - |
- |
Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.3 | guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
? | |
| 3.5.4.3 | guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.3 | Oryctolagus cuniculus | - |
rabbit | - |
| 3.5.4.3 | Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.3 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.4.3 | liver | - |
Rattus norvegicus | - |
| 3.5.4.3 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.3 | 25.2 | - |
liver enzyme | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.3 | 6-thioguanine + H2O | - |
Oryctolagus cuniculus | 6-thioxanthine + NH3 | - |
? | |
| 3.5.4.3 | 8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
| 3.5.4.3 | guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
? | |
| 3.5.4.3 | guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.3 | monomer | - |
Rattus norvegicus |
| 3.5.4.3 | monomer | 1 * 55000, gel filtration, SDS-PAGE | Oryctolagus cuniculus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.3 | 20 | 50 | activation energy linear | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.3 | 50 | - |
- |
Rattus norvegicus |
| 3.5.4.3 | 50 | - |
relatively stable up to 50°C | Oryctolagus cuniculus |
| 3.5.4.3 | 55 | - |
42% loss of enzyme activity substained upon incubation for 25 min | Oryctolagus cuniculus |
| 3.5.4.3 | 55 | - |
loss of catalytic activity upon incubation for 20 min | Oryctolagus cuniculus |
| 3.5.4.3 | 60 | - |
- |
Rattus norvegicus |
| 3.5.4.3 | 60 | - |
irreversible denaturation upon incubation for 25 min | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.3 | 6 | - |
8-azaguanine as substrate | Oryctolagus cuniculus |
| 3.5.4.3 | 6.8 | - |
pH optimum is independent of the buffer system | Oryctolagus cuniculus |
| 3.5.4.3 | 7.7 | - |
- |
Oryctolagus cuniculus |
| 3.5.4.3 | 8 | - |
- |
Rattus norvegicus |
| 3.5.4.3 | 8 | - |
- |
Oryctolagus cuniculus |
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