BRENDA - Enzyme Database show

Substrate binding by blasticidin S deaminase, an aminohydrolase for novel 4-aminopyrimidine nucleosides

Yamaguchi, I.; Sheto, H.; Misato, T.; Pestic. Biochem. Physiol. 25, 54-62 (1986)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.23
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.23
4-deaminohydroxycytomycin
competitive inhibition
Aspergillus terreus
3.5.4.23
acetylcytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
acetyldeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
caproylcytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
cytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
deaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
deaminohydroxyblasticidin S methylester
-
Aspergillus terreus
3.5.4.23
deaminomercaptoblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
dihydrocytomycin
competitive inhibition
Aspergillus terreus
3.5.4.23
dihydrodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
pyrimidinodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
uracinine
competitive inhibition
Aspergillus terreus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.23
0.021
-
blasticidin S
-
Aspergillus terreus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.23
blasticidin S + H2O
Bacillus sp. (in: Bacteria)
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Streptomyces sp.
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.23
Aspergillus fumigatus
-
-
-
3.5.4.23
Aspergillus terreus
-
-
-
3.5.4.23
Bacillus sp. (in: Bacteria)
-
-
-
3.5.4.23
Streptomyces sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.23
-
Aspergillus terreus
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.23
-20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months
Aspergillus terreus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.23
acetylblasticidin S + H2O
-
209570
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
alanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Bacillus sp. (in: Bacteria)
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Streptomyces sp.
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S methylester + H2O
only slightly deaminated
209570
Aspergillus terreus
deaminohydroxyblasticidin S methylester + NH3
-
-
-
?
3.5.4.23
cytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
cytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytosinine + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
demethylblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
deoxycytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
dihydroblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
dihydrocytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
additional information
cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
phenylalanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
pyrimidinoblasticidin S + H2O
-
209570
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S + NH3
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.23
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.23
4-deaminohydroxycytomycin
competitive inhibition
Aspergillus terreus
3.5.4.23
acetylcytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
acetyldeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
caproylcytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
cytosinine
competitive inhibition
Aspergillus terreus
3.5.4.23
deaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
deaminohydroxyblasticidin S methylester
-
Aspergillus terreus
3.5.4.23
deaminomercaptoblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
dihydrocytomycin
competitive inhibition
Aspergillus terreus
3.5.4.23
dihydrodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
pyrimidinodeaminohydroxyblasticidin S
competitive inhibition
Aspergillus terreus
3.5.4.23
uracinine
competitive inhibition
Aspergillus terreus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.23
0.021
-
blasticidin S
-
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.23
blasticidin S + H2O
Bacillus sp. (in: Bacteria)
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Streptomyces sp.
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.23
-
Aspergillus terreus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.23
-20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.23
acetylblasticidin S + H2O
-
209570
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
alanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Bacillus sp. (in: Bacteria)
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Streptomyces sp.
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209570
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S methylester + H2O
only slightly deaminated
209570
Aspergillus terreus
deaminohydroxyblasticidin S methylester + NH3
-
-
-
?
3.5.4.23
cytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
cytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytosinine + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
demethylblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
deoxycytidine + H2O
-
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
dihydroblasticidin S + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
dihydrocytomycin + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
additional information
cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates
209570
Aspergillus terreus
?
-
-
-
-
3.5.4.23
phenylalanylcytosinine + H2O
-
209570
Aspergillus terreus
?
-
-
-
?
3.5.4.23
pyrimidinoblasticidin S + H2O
-
209570
Aspergillus terreus
pyrimidinodeaminohydroxyblasticidin S + NH3
-
-
-
?