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Literature summary extracted from

  • Yamaguchi, I.; Sheto, H.; Misato, T.
    Substrate binding by blasticidin S deaminase, an aminohydrolase for novel 4-aminopyrimidine nucleosides (1986), Pestic. Biochem. Physiol., 25, 54-62.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.23 Gougerotin enzyme induced by Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.23 4-deaminohydroxycytomycin competitive inhibition Aspergillus terreus
3.5.4.23 acetylcytosinine competitive inhibition Aspergillus terreus
3.5.4.23 acetyldeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
3.5.4.23 caproylcytosinine competitive inhibition Aspergillus terreus
3.5.4.23 cytosinine competitive inhibition Aspergillus terreus
3.5.4.23 deaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
3.5.4.23 deaminohydroxyblasticidin S methylester
-
 Aspergillus terreus
3.5.4.23 deaminomercaptoblasticidin S competitive inhibition Aspergillus terreus
3.5.4.23 dihydrocytomycin competitive inhibition Aspergillus terreus
3.5.4.23 dihydrodeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
3.5.4.23 pyrimidinodeaminohydroxyblasticidin S competitive inhibition Aspergillus terreus
3.5.4.23 uracinine competitive inhibition Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.23 0.021
-
 blasticidin S
-
 Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.23 blasticidin S + H2O Bacillus sp. (in: Bacteria)
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Streptomyces sp.
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus terreus
-
 deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O Aspergillus fumigatus
-
 deaminohydroxyblasticidin S + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.23 Aspergillus fumigatus
-
-
-
3.5.4.23 Aspergillus terreus
-
-
-
3.5.4.23 Bacillus sp. (in: Bacteria)
-
-
-
3.5.4.23 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.23
-
 Aspergillus terreus

Storage Stability

EC Number Storage Stability Organism
3.5.4.23 -20°C, 40% glycerol-5 mM Tris-HCl buffer, pH 7.5, 5 mM 2-mercaptoethanol, no detectable loss of activity over a period of 3 months Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.23 acetylblasticidin S + H2O
-
 Aspergillus terreus acetyldeaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 alanylcytosinine + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Bacillus sp. (in: Bacteria) deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Streptomyces sp. deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus terreus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S + H2O
-
 Aspergillus fumigatus deaminohydroxyblasticidin S + NH3
-
 ?
3.5.4.23 blasticidin S methylester + H2O only slightly deaminated Aspergillus terreus deaminohydroxyblasticidin S methylester + NH3
-
 ?
3.5.4.23 cytidine + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 cytomycin + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 cytosinine + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
3.5.4.23 demethylblasticidin S + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 deoxycytidine + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 dihydroblasticidin S + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 dihydrocytomycin + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 gougerotin + H2O only slightly deaminated Aspergillus terreus ?
-
 ?
3.5.4.23 additional information cytosine, CMP, d-CMP, adenine, guanine and their nucleosides and nucleotides are no substrates Aspergillus terreus ?
-
 ?
3.5.4.23 phenylalanylcytosinine + H2O
-
 Aspergillus terreus ?
-
 ?
3.5.4.23 pyrimidinoblasticidin S + H2O
-
 Aspergillus terreus pyrimidinodeaminohydroxyblasticidin S + NH3
-
 ?