BRENDA - Enzyme Database show

Isolation and purification of blasticidin S deaminase from Aspergillus terreus

Yamaguchi, I.; Shibata, H.; Seto, H.; Misato, T.; J. Antibiot. 28, 7-14 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.23
2-mercaptoethanol
protecting enzyme
Aspergillus terreus
3.5.4.23
blasticidin S
produces far more enzyme when grown in presence
Aspergillus terreus
3.5.4.23
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.23
CuCl2
-
Aspergillus terreus
3.5.4.23
HgCl2
-
Aspergillus terreus
3.5.4.23
p-chloromercuribenzoate
-
Aspergillus terreus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.23
29600
-
sedimentation analysis
Aspergillus terreus
3.5.4.23
30000
-
SDS-PAGE
Aspergillus terreus
3.5.4.23
46000
-
gel filtration
Aspergillus terreus
3.5.4.23
55000
-
gel filtration, buffer without sodium chloride
Aspergillus terreus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.23
blasticidin S + H2O
Aspergillus flavus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ochraceus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ustus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus flavipes
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus flavipes NHL 5013
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ustus 1004
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus NHL 5009
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ochraceus 67-MI-87
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus NHL 5037
-
deaminohydroxyblasticidin S + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.23
Aspergillus flavipes
-
NHL 5013
-
3.5.4.23
Aspergillus flavipes NHL 5013
-
NHL 5013
-
3.5.4.23
Aspergillus flavus
-
ATCC 9643
-
3.5.4.23
Aspergillus fumigatus
-
NHL 5009
-
3.5.4.23
Aspergillus fumigatus NHL 5009
-
NHL 5009
-
3.5.4.23
Aspergillus ochraceus
-
67-MI-87
-
3.5.4.23
Aspergillus ochraceus 67-MI-87
-
67-MI-87
-
3.5.4.23
Aspergillus terreus
-
NHL 5037
-
3.5.4.23
Aspergillus terreus NHL 5037
-
NHL 5037
-
3.5.4.23
Aspergillus ustus
-
1004
-
3.5.4.23
Aspergillus ustus 1004
-
1004
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.23
-
Aspergillus terreus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.23
220.5
-
-
Aspergillus terreus
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.23
-20°C, 50% glycerol 5 mM mercaptoethanol, no appreciable loss of activity after 3 months
Aspergillus terreus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.23
acetylblasticidin S + H2O
-
209567
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
acetylblasticidin S + H2O
-
209567
Aspergillus terreus NHL 5037
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ochraceus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ustus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavipes
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavipes NHL 5013
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ustus 1004
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus fumigatus NHL 5009
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ochraceus 67-MI-87
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus terreus NHL 5037
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
cytidine + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytomycin + H2O
-
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytomycin + H2O
-
209567
Aspergillus terreus NHL 5037
?
-
-
-
?
3.5.4.23
deoxycytidine + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209567
Aspergillus terreus NHL 5037
?
-
-
-
?
3.5.4.23
additional information
cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates
209567
Aspergillus terreus
?
-
-
-
-
3.5.4.23
additional information
cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates
209567
Aspergillus terreus NHL 5037
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.23
60
70
-
Aspergillus terreus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.23
20
80
-
Aspergillus terreus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.23
60
80
reaction proceeds most rapidly at 60-70°C, occurs only during a short initial period at 80°C
Aspergillus terreus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.23
10
-
Tris-HCl, glycinate or bicarbonate buffer
Aspergillus terreus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.23
5
12
-
Aspergillus terreus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.23
4.5
-
rapid inactivation below
Aspergillus terreus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.23
2-mercaptoethanol
protecting enzyme
Aspergillus terreus
3.5.4.23
blasticidin S
produces far more enzyme when grown in presence
Aspergillus terreus
3.5.4.23
Gougerotin
enzyme induced by
Aspergillus terreus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.23
CuCl2
-
Aspergillus terreus
3.5.4.23
HgCl2
-
Aspergillus terreus
3.5.4.23
p-chloromercuribenzoate
-
Aspergillus terreus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.23
29600
-
sedimentation analysis
Aspergillus terreus
3.5.4.23
30000
-
SDS-PAGE
Aspergillus terreus
3.5.4.23
46000
-
gel filtration
Aspergillus terreus
3.5.4.23
55000
-
gel filtration, buffer without sodium chloride
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.23
blasticidin S + H2O
Aspergillus flavus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ochraceus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ustus
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus flavipes
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus flavipes NHL 5013
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ustus 1004
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus fumigatus NHL 5009
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus ochraceus 67-MI-87
-
deaminohydroxyblasticidin S + NH3
-
-
?
3.5.4.23
blasticidin S + H2O
Aspergillus terreus NHL 5037
-
deaminohydroxyblasticidin S + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.23
-
Aspergillus terreus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.23
220.5
-
-
Aspergillus terreus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.23
-20°C, 50% glycerol 5 mM mercaptoethanol, no appreciable loss of activity after 3 months
Aspergillus terreus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.23
acetylblasticidin S + H2O
-
209567
Aspergillus terreus
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
acetylblasticidin S + H2O
-
209567
Aspergillus terreus NHL 5037
acetyldeaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus terreus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ochraceus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus fumigatus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ustus
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavipes
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus flavipes NHL 5013
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ustus 1004
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus fumigatus NHL 5009
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus ochraceus 67-MI-87
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
blasticidin S + H2O
-
209567
Aspergillus terreus NHL 5037
deaminohydroxyblasticidin S + NH3
-
-
-
?
3.5.4.23
cytidine + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytomycin + H2O
-
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
cytomycin + H2O
-
209567
Aspergillus terreus NHL 5037
?
-
-
-
?
3.5.4.23
deoxycytidine + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209567
Aspergillus terreus
?
-
-
-
?
3.5.4.23
gougerotin + H2O
only slightly deaminated
209567
Aspergillus terreus NHL 5037
?
-
-
-
?
3.5.4.23
additional information
cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates
209567
Aspergillus terreus
?
-
-
-
-
3.5.4.23
additional information
cytosine, cytosinine, CMP, d-CMP, adenine, adenosine, AMP, guanine, guanosine, GMP or other purine bases or their nucleosides are no substrates
209567
Aspergillus terreus NHL 5037
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.23
60
70
-
Aspergillus terreus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.23
20
80
-
Aspergillus terreus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.4.23
60
80
reaction proceeds most rapidly at 60-70°C, occurs only during a short initial period at 80°C
Aspergillus terreus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.23
10
-
Tris-HCl, glycinate or bicarbonate buffer
Aspergillus terreus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.23
5
12
-
Aspergillus terreus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.23
4.5
-
rapid inactivation below
Aspergillus terreus