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Literature summary extracted from

  • Takikawa, S.; Kitayama-Yokokawa, C.; Tsusue, M.
    Pterin deaminase from Bacillus megaterium (1979), J. Biochem., 85, 785-790.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.4.11 freezing and thawing causes a severe loss of activity Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.11 4-chloromercuribenzoate 1 mM, 69% inhibition Priestia megaterium
3.5.4.11 8-Azaguanine 1 mM, 58% inhibition Priestia megaterium
3.5.4.11 KCN 10 mM, 12% inhibition Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.11 1.3
-
 2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid 25°C, pH 7.0 Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.11 110000
-
 gel filtration Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.11 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.11 partial Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.11 0.017
-
 25°C, pH 7.0 Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
3.5.4.11 -20°C or -80°C, the activity is maintained for several months Priestia megaterium
3.5.4.11 4°C, the activity decreases about 80% after standing overnight Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.11 2-amino-3,5-dihydropteridine-4,6-dione + H2O i.e. xanthopterin Priestia megaterium 1,5-dihydropteridine-2,4,6(3H)-trione + NH3
-
 ?
3.5.4.11 2-amino-4-hydroxypteridine + H2O i.e. pterin Priestia megaterium 2,4-dihydroxypteridine + NH3 i.e. lumazine ?
3.5.4.11 2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid + H2O i.e. pterin 6-carboxylate Priestia megaterium 2,4-dioxo-1,2,3,4-tetrahydropteridine-6-carboxylic acid + NH3 i.e. 2,4-dioxo-pteridine-6-carboxylate ?
3.5.4.11 2-amino-6-hydroxypteridin-4(3H)-one + H2O i.e. 6-hydroxypterin Priestia megaterium 6-hydroxypteridine-2,4(1H,3H)-dione + NH3
-
 ?
3.5.4.11 2-amino-6-methylpteridin-4(3H)-one + H2O i.e. 6-methylpterin Priestia megaterium 6-methylpteridine-2,4(1H,3H)-dione + NH3
-
 ?
3.5.4.11 2-amino-6-[(1R,2S)-1,2-dihydroxypropyl]pteridin-4(8H)-one + H2O i.e. biopterin Priestia megaterium 6-[(1R,2S)-1,2-dihydroxypropyl]pteridine-2,4(1H,3H)-dione + NH3
-
 ?
3.5.4.11 2-amino-6-[(2S)-2-hydroxypropanoyl]-7,8-dihydropteridin-4(1H)-one + H2O i.e. sepiapterin Priestia megaterium 6-[(2S)-2-hydroxypropanoyl]-7,8-dihydropteridine-2,4(1H,3H)-dione + NH3
-
 ?
3.5.4.11 2-amino-7-methylpteridin-4(3H)-one + H2O i.e. 7-methylpterin Priestia megaterium 7-methylpteridine-2,4(1H,3H)-dione + NH3
-
 ?
3.5.4.11 additional information guanine, 7-carboxypterin, leucopterin, isoxanthopterin, and 6-methylisoxanthopterin do not serve as substrates Priestia megaterium ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.11 7.3
-
 substrate: 2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.11 5.5 8 pH 5.5: about 65% of maximal activity, pH 8.0: about 85% of maximal activity Priestia megaterium