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Literature summary extracted from

  • Porter, D.J.T.; Austin, E.A.
    Cytosine deaminase the roles of divalent metal ions in catalysis (1993), J. Biol. Chem., 268, 24005-24011.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.1 medicine oral 5-fluorocytosine in combination with selective delivery of CDase to cancerous cells by selectively introducing the bacterial CDase gene into the genome Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.1 CDase gene cloned into pBS+ vector, gentically engineered Escherichia coli strain that overexpresses the codBA operon on a multicopy plasmid Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 Cu2+ Fe2+CDase Escherichia coli
3.5.4.1 H2O2 only Fe2+CDase, Mn2+CDase, Co2+CDase and Zn2+CDase are not inhibited Escherichia coli
3.5.4.1 o-phenanthroline
-
 Escherichia coli
3.5.4.1 Zn2+ Fe2+CDase Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 0.22
-
 cytosine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.1 Co2+ Mn2+ reconstitutes apoenzyme after removal of Fe2+ Escherichia coli
3.5.4.1 Fe2+
-
 Escherichia coli
3.5.4.1 MnCl2 Mn2+ reconstitutes apoenzyme after removal of Fe2+ Escherichia coli
3.5.4.1 Zn2+ Mn2+ reconstitutes apoenzyme after removal of Fe2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 48000
-
 4 * 48000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 2
-
 + 0.025 mM Cu(C2H3O2) Escherichia coli
3.5.4.1 2.6
-
 + 0.025 mM HgCl2 Escherichia coli
3.5.4.1 2.6
-
 + 0.025 mM Pb(C2H3O2) Escherichia coli
3.5.4.1 2.7
-
 + 0.025 mM CrCl2 Escherichia coli
3.5.4.1 2.9
-
 apoCDase, + 0.025 SnCl2 Escherichia coli
3.5.4.1 3.1
-
 + 0.025 mM Cu(C2H3O2) Escherichia coli
3.5.4.1 3.2
-
 + 0.025 mM CaCl2 Escherichia coli
3.5.4.1 4
-
 + 0.025 mM NiCl2, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 5.6
-
 + 0.025 mM NiCl2 Escherichia coli
3.5.4.1 8.3
-
 + 0.025 mM Pb(C2H3O2), additional incubation of 0.025 mM Fe2+ Escherichia coli
3.5.4.1 11
-
 + 0.025 mM HgCl2, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 12
-
 + 0.025 mM Zn(C2H3O2), additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 13
-
 + 0.025 mM Zn(C2H3O2) Escherichia coli
3.5.4.1 21
-
 + 0.025 mM CoSO4, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 26
-
 + 0.025 mM CoSO4 Escherichia coli
3.5.4.1 27
-
-
 Escherichia coli
3.5.4.1 45
-
 + 0.025 mM MnSO4 Escherichia coli
3.5.4.1 62
-
 + 0.025 mM MnSO4, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 65
-
 + 0.025 mM FeSO4, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 87
-
 + 0.025 mM FeSO4 Escherichia coli
3.5.4.1 87
-
 + 0.025 mM CrCl2, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 87
-
 apoCDase, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 88
-
 + 0.025 mM CaCl2, additional incubation with 0.025 mM Fe2+ Escherichia coli
3.5.4.1 94
-
 + 0.025 SnCl2, additional incubation with 0.025 mM Fe2+ Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.4.1 5°C, loses 40% of its activity after 2 weeks, lost activity can be restored by treatment with 0.05 mM FeSO4 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O
-
 Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.1 tetramer 4 * 48000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.1 73
-
 extremely thermostable Escherichia coli